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SERPINE1 elisa kit :: PAI-1 functional assay ELISA Kit

Scan QR to view Datasheet Catalog #    MBS135492
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1 Kit  /  $540 +1 FREE 8GB USB
5 Kits  /  $1,930 +2 FREE 8GB USB
 
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 Product Name   

PAI-1 functional assay (SERPINE1), ELISA Kit

★Popular Item★
 Also Known As   

Active human PAI-1 functional assay ELISA Kit

 Product Gene Name   

SERPINE1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 7; NC_000007.13 (100770370..100782547). Location: 7q22.1
 OMIM    173360
 3D Structure    ModBase 3D Structure for P05121
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 Host    Multiple
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 Specificity    This assay recognizes natural active human PAI-1. Pooled normal plasma from Sheep was assayed, and no significant cross-reactivity was observed. Pooled normal plasma from Mouse resulted in significant color development. The following factors were tested for cross-reactivity.
 Form/Format    Complete Kit
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 Samples    Human plasma
 Sensitivity    The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.161-0.178) and calculating the corresponding concentration. The MDD was 0.385 U/ml.
 Intra-assay Precision    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
 Inter-assay Precision    Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SERPINE1 purchase    MBS135492 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple PAI-1 functional assay (SERPINE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE1, with an estimated sensitivity of The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.161-0.178) and calculating the corresponding concentration. The MDD was 0.385 U/ml. The ELISA analytical biochemical technique of the MBS135492 kit is based on SERPINE1 antibody-SERPINE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SERPINE1 elisa kit

   Intended Uses: This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 in human plasma. For research use only.

Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate or be detected. Unbound PAI-1 samples are aspirated and an anti-PAI-1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.

Background: PAI-1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases [1,2]. Levels of PAI-1 increase with age [5] and are elevated in conditions such as normal pregnancy [3] and sepsis [4].
 Product Categories/Family for SERPINE1 elisa kit    Plasminogen Activator Inhibitor 1 (PAI-1); PAI-1 ELISA Kits
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 Typical Testing Data/Standard Curve (for reference only) of SERPINE1 elisa kit    SERPINE1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS135492. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SERPINE1. It may not necessarily be applicable to this product.
 NCBI GI #    10835159
 NCBI GeneID    5054
 NCBI Accession #    NP_000593.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000602.4 [Other Products]
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 UniProt Primary Accession #    P05121 [Other Products]
 UniProt Secondary Accession #    B7Z4S0; F8WD53 [Other Products]
 UniProt Related Accession #    P05121 [Other Products]
 Molecular Weight    45,060 Da
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 NCBI Official Full Name    plasminogen activator inhibitor 1
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
 NCBI Official Symbol    SERPINE1 [Similar Products]
 NCBI Official Synonym Symbols   
PAI; PAI1; PAI-1; PLANH1
[Similar Products]
 NCBI Protein Information    plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
 UniProt Protein Name    Plasminogen activator inhibitor 1
 UniProt Synonym Protein Names   
Endothelial plasminogen activator inhibitor; Serpin E1
 Protein Family    Plasminogen activator inhibitor
 UniProt Gene Name    SERPINE1 [Similar Products]
 UniProt Synonym Gene Names    PAI1; PLANH1; PAI; PAI-1 [Similar Products]
 UniProt Entry Name    PAI1_HUMAN
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 NCBI Summary for SERPINE1    This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for SERPINE1    SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell migration; negative regulation of cell adhesion mediated by integrin; platelet activation; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
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 Research Articles on SERPINE1    1. The prevalence of the PAI-1 5G/5G genotype in patients with APS and thrombosis was significantly lower than in those with SLE without APS or thrombosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SERPINE1 elisa kitPathways associated with SERPINE1 elisa kit
 Reference Product  PubMed Publications
 PLG elisa kit  >41 publications with SERPINE1 and PLG
 PLAT elisa kit  >21 publications with SERPINE1 and PLAT
 PLAU elisa kit  >10 publications with SERPINE1 and PLAU
 MMP3 elisa kit  >10 publications with SERPINE1 and MMP3
 IGFBP5 elisa kit  >7 publications with SERPINE1 and IGFBP5
 VTN elisa kit  >5 publications with SERPINE1 and VTN
 LRP1B elisa kit  >2 publications with SERPINE1 and LRP1B
 LRP2 elisa kit  >1 publications with SERPINE1 and LRP2
 ACTN4 elisa kit  >1 publications with SERPINE1 and ACTN4
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies  BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
Diseases associated with SERPINE1 elisa kitOrgans/Tissues associated with SERPINE1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2981 publications with SERPINE1 and Cardiovascular Diseases
 Neoplasms Antibodies  >1328 publications with SERPINE1 and Neoplasms
 Thrombosis Antibodies  >1295 publications with SERPINE1 and Thrombosis
 Inflammation Antibodies  >1140 publications with SERPINE1 and Inflammation
 Heart Diseases Antibodies  >977 publications with SERPINE1 and Heart Diseases
 Necrosis Antibodies  >775 publications with SERPINE1 and Necrosis
 Atherosclerosis Antibodies  >721 publications with SERPINE1 and Atherosclerosis
 Hypertension Antibodies  >651 publications with SERPINE1 and Hypertension
 Fibrosis Antibodies  >603 publications with SERPINE1 and Fibrosis
 Myocardial Infarction Antibodies  >485 publications with SERPINE1 and Myocardial Infarction
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >2628 publications with SERPINE1 and Vascular
 Liver Antibodies  >539 publications with SERPINE1 and Liver
 Lung Antibodies  >511 publications with SERPINE1 and Lung
 Embryonic Tissue Antibodies  >321 publications with SERPINE1 and Embryonic Tissue
 Umbilical Cord Antibodies  >206 publications with SERPINE1 and Umbilical Cord
 Skin Antibodies  >179 publications with SERPINE1 and Skin
 Placenta Antibodies  >120 publications with SERPINE1 and Placenta
 Intestine Antibodies  >67 publications with SERPINE1 and Intestine
 Pancreas Antibodies  >39 publications with SERPINE1 and Pancreas
 Amniotic Fluid Antibodies  >12 publications with SERPINE1 and Amniotic Fluid
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