Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow SERPINE1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • BIM Antibody
 • Chk1 (Phospho-Ser317) Antibody
 • SURF1 Antibody
 • PKCD (Ab-64) Antibody
 • Coagulation Factor III (Phospho-Ser290) (F3) Antibody
 • Teichoic Acid Antibody (TA) ELISA Kit
 • a1-Acid Glycoprotein (a1-AGP) ELISA Kit
 • Nitric Oxide Synthase (NOS) ELISA Kit
 • Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit
 • Colicin ELISA Kit
 • Sperm Protein 17 (SP17) ELISA Kit
 • P Selectin (P-SELECTIN) ELISA Kit
 • Aquaporin 3, Gill Blood Group (AQP-3) ELISA Kit
 • Soluble Cluster of Differentiation 40 Ligand (SCD40L) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

SERPINE1 elisa kit :: PAI-1 total antigen assay ELISA Kit

Scan QR to view Datasheet Catalog #    MBS135534
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1 Kit  /  $540 +1 FREE 8GB USB
5 Kits  /  $1,930 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PAI-1 total antigen assay (SERPINE1), ELISA Kit

 Also Known As   

Mouse PAI-1 total antigen assay ELISA Kit

 Product Gene Name   

SERPINE1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 5; NC_000071.6 (137061504..137072272, complement). Location: 5 G2; 5
 3D Structure    ModBase 3D Structure for P22777
Section Bar
 Host    Multiple
Section Bar
 Specificity    This assay recognizes natural total mouse PAI-1. Pooled normal plasma from Rat, Human, Pig, Dog, and Sheep were assayed, and no significant cross-reactivity was observed. Pooled normal plasma from Rabbit resulted in significant color development.
 Form/Format    Complete Kit
Section Bar
 Samples    Mouse plasma
 Sensitivity    The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.072-0.078) and calculating the corresponding concentration. The MDD was 0.006 ng/ml.
 Intra-assay Precision    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
 Inter-assay Precision    Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
Section Bar
 Preparation and Storage    Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SERPINE1 purchase    MBS135534 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple PAI-1 total antigen assay (SERPINE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE1, with an estimated sensitivity of The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.072-0.078) and calculating the corresponding concentration. The MDD was 0.006 ng/ml. The ELISA analytical biochemical technique of the MBS135534 kit is based on SERPINE1 antibody-SERPINE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for SERPINE1 elisa kit

   Intended Uses: Mouse PAI-1 total assay is intended for the quantitative determination of total plasminogen activator inhibitor type 1 in mouse plasma. For research use only.

Principle of the Assay: Mouse PAI-1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. Any unbound PAI-1 is washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI-1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI-1 in the sample.

Background: Plasminogen activator inhibitor-1 (PAI-1) is a central regulator of the blood fibrinolytic system [2]. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding [3].
 Product Categories/Family for SERPINE1 elisa kit    Plasminogen Activator Inhibitor 1 (PAI-1); PAI-1 ELISA Kits
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of SERPINE1 elisa kit    SERPINE1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS135534. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SERPINE1. It may not necessarily be applicable to this product.
 NCBI GI #    129577
 NCBI GeneID    18787
 NCBI Accession #    P22777.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P22777 [Other Products]
 UniProt Related Accession #    P22777 [Other Products]
 Molecular Weight    45,170 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Plasminogen activator inhibitor 1
 NCBI Official Synonym Full Names    serine (or cysteine) peptidase inhibitor, clade E, member 1
 NCBI Official Symbol    Serpine1 [Similar Products]
 NCBI Official Synonym Symbols   
PAI1; PAI-1; Planh1
[Similar Products]
 NCBI Protein Information    plasminogen activator inhibitor 1; PAI; serpin E1; plasminogen activator inhibitor, type I; endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI)
 UniProt Protein Name    Plasminogen activator inhibitor 1
 UniProt Synonym Protein Names   
Endothelial plasminogen activator inhibitor; Serpin E1
 Protein Family    Plasminogen activator inhibitor
 UniProt Gene Name    Serpine1 [Similar Products]
 UniProt Synonym Gene Names    Mr1; Pai1; Planh1; PAI; PAI-1 [Similar Products]
 UniProt Entry Name    PAI1_MOUSE
Section Bar
 UniProt Comments for SERPINE1    SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; protease inhibitor activity; receptor binding

Biological Process: positive regulation of keratinocyte migration; positive regulation of blood coagulation; wound healing; negative regulation of peptidase activity; positive regulation of coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; regulation of angiogenesis; positive regulation of receptor-mediated endocytosis; regulation of cell proliferation; positive regulation of interleukin-8 production; positive regulation of angiogenesis; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; regulation of receptor activity; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; positive regulation of inflammatory response
Table BarTOPTable Bar
 Research Articles on SERPINE1    1. mRNA expression profiling in diabetic endothelial cells revealed significant and persistent up-regulation of Serpine1 encoding PAI-1
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with SERPINE1 elisa kitOrgans/Tissues associated with SERPINE1 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies  BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >539 publications with SERPINE1 and Connective Tissue
 Lung Antibodies  >511 publications with SERPINE1 and Lung
 Embryonic Tissue Antibodies  >321 publications with SERPINE1 and Embryonic Tissue
 Bone Marrow Antibodies  >98 publications with SERPINE1 and Bone Marrow
 Eye Antibodies  >92 publications with SERPINE1 and Eye
 Joint Antibodies  >88 publications with SERPINE1 and Joint
 Spleen Antibodies  >22 publications with SERPINE1 and Spleen
 Testis Antibodies  >21 publications with SERPINE1 and Testis
 Mammary Gland Antibodies  >19 publications with SERPINE1 and Mammary Gland
 Thymus Antibodies  >3 publications with SERPINE1 and Thymus
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions