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CD40LG elisa kit :: Pig CD40 ligand ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886234
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD40 ligand (CD40LG), ELISA Kit

★Popular Item★
 Also Known As   

Pig CD40 ligand ELISA Kit

 Product Synonym Names    CD40 ligand; CD40-L; Tumor necrosis factor ligand superfamily member 5; CD40LG; CD40L; TNFSF5; CD154
 Product Gene Name   

CD40LG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886234 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q95MQ5
 Species Reactivity    Pig
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 Specificity    Recombinant and natural Pig CD40 ligand,CD40-L
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 Samples    Serum, plasma, tissue homogenates,cell culture supernates or other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    0.312-20 ng/mL
 Intra-assay Precision    Intra-Assay CV: <=5.3%
 Inter-assay Precision    Inter-Assay CV: <=8.2%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CD40LG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CD40LG purchase    MBS2886234 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD40 ligand (CD40LG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20 ng/mL in biological research samples containing CD40LG. The ELISA analytical biochemical technique of the MBS2886234 kit is based on CD40LG antibody-CD40LG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD40LG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD40LG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates,cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CD40LG elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates,cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CD40LG elisa kit    CD40LG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2886234. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
 NCBI GI #    47523056
 NCBI GeneID    397231
 NCBI Accession #    NP_999291.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214126.1 [Other Products]
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 UniProt Primary Accession #    Q95MQ5 [Other Products]
 UniProt Related Accession #    Q95MQ5 [Other Products]
 Molecular Weight    28,869 Da
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 NCBI Official Full Name    CD40 ligand
 NCBI Official Symbol    CD40LG [Similar Products]
 NCBI Official Synonym Symbols   
CD40L; TNFSF5
[Similar Products]
 NCBI Protein Information    CD40 ligand
 UniProt Protein Name    CD40 ligand
 UniProt Synonym Protein Names   
Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154
 Protein Family    CD40 ligand
 UniProt Gene Name    CD40LG [Similar Products]
 UniProt Synonym Gene Names    CD40L; TNFSF5; CD40-L [Similar Products]
 UniProt Entry Name    CD40L_PIG
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 UniProt Comments for CD40LG    Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.
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 Research Articles on CD40LG    1. CD40 ligand has a role in inducing endothelial dysfunction in human and porcine coronary artery endothelial cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CD40LG elisa kitPathways associated with CD40LG elisa kit
 Reference Product  PubMed Publications
 CD40 elisa kit  >40 publications with CD40LG and CD40
 ICOS elisa kit  >6 publications with CD40LG and ICOS
 IL2 elisa kit  >2 publications with CD40LG and IL2
 VCAM1 elisa kit  >1 publications with CD40LG and VCAM1
 CD27 elisa kit  >1 publications with CD40LG and CD27
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
Diseases associated with CD40LG elisa kit
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >13 publications with CD40LG and Autoimmune Diseases
 Inflammation Antibodies  >12 publications with CD40LG and Inflammation
 Necrosis Antibodies  >9 publications with CD40LG and Necrosis
 Hyper-IgM Immunodeficiency Syndrome, Type 1 Antibodies  >6 publications with CD40LG and Hyper-IgM Immunodeficiency Syndrome, Type 1
 Cardiovascular Diseases Antibodies  >6 publications with CD40LG and Cardiovascular Diseases
 Anemia Antibodies  >4 publications with CD40LG and Anemia
 Myocardial Infarction Antibodies  >3 publications with CD40LG and Myocardial Infarction
 Hypersensitivity Antibodies  >3 publications with CD40LG and Hypersensitivity
 Myocardial Ischemia Antibodies  >3 publications with CD40LG and Myocardial Ischemia
 Pneumonia Antibodies  >3 publications with CD40LG and Pneumonia
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