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IFN-alpha elisa kit :: Pig Interferon alpha, IFN-alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700009
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon alpha, IFN-alpha, ELISA Kit

★Popular Item★
 Also Known As   

Pig Interferon alpha, IFN-alpha ELISA Kit

 Product Synonym Names    Pig Interferon alpha; IFN-alpha ELISA Kit; Interferon alpha; IFN-alpha
 Product Gene Name   

IFN-alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700009 Testing Data    Testing Data PDF
 OMIM    147553
 3D Structure    ModBase 3D Structure for P05000
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 Species Reactivity    Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of pig IFN-alpha. No significant cross-reactivity or interference between pig IFN-alpha and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    7.8 pg/ml-500 pg/ml
 Sensitivity    <1.95pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IFN-alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IFN-alpha purchase    MBS700009 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon alpha, IFN-alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 pg/ml-500 pg/ml in biological research samples containing IFN-alpha, with an estimated sensitivity of <1.95pg/ml. The ELISA analytical biochemical technique of the MBS700009 kit is based on IFN-alpha antibody-IFN-alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFN-alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFN-alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IFN-alpha elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IFN-alpha elisa kit    IFN-alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700009. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IFN-alpha. It may not necessarily be applicable to this product.
 NCBI GI #    386800
 NCBI GeneID    3467
 NCBI Accession #    AAA52724.1 [Other Products]
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 UniProt Primary Accession #    P05000 [Other Products]
 UniProt Secondary Accession #    Q13168; Q5U802; Q5VWD0; Q7M4P5 [Other Products]
 UniProt Related Accession #    P05000 [Other Products]
 Molecular Weight    22,319 Da
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 NCBI Official Full Name    interferon-alpha
 NCBI Official Synonym Full Names    interferon, omega 1
 NCBI Official Symbol    IFNW1 [Similar Products]
 NCBI Protein Information    interferon omega-1; interferon alpha-II-1; IFN-omega 1, interferon omega-1
 UniProt Protein Name    Interferon omega-1
 UniProt Synonym Protein Names   
Interferon alpha-II-1
 UniProt Gene Name    IFNW1 [Similar Products]
 UniProt Entry Name    IFNW1_HUMAN
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 UniProt Comments for IFN-alpha    IFNW1: Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
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 Research Articles on IFN-alpha    1. Studies indicate that upregulation of the type I interferon protein signature has added additional markers of disease activity and insight into the pathogenesis of the disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IFN-alpha elisa kitPathways associated with IFN-alpha elisa kit
 Reference Product  PubMed Publications
 IFNA2 elisa kit  >1 publications with IFN-alpha and IFNA2
 MTAP elisa kit  >1 publications with IFN-alpha and MTAP
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
Diseases associated with IFN-alpha elisa kit
 Disease Name  Pubmed Publications
 Skin Neoplasms Antibodies  >1 publications with IFN-alpha and Skin Neoplasms
 Immune System Diseases Antibodies  >1 publications with IFN-alpha and Immune System Diseases
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