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PI elisa kit :: Pig Proinsulin, PI ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700914 PI elisa kit
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proinsulin, PI, ELISA Kit

★Popular Item★
 Also Known As   

Pig Proinsulin, PI ELISA Kit

 Product Synonym Names    Pig Proinsulin; PI ELISA Kit; Proinsulin; PI
 Product Gene Name   

PI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613370
 3D Structure    ModBase 3D Structure for P01308
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 Species Reactivity    Pig
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 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PI purchase    MBS700914 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proinsulin, PI, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PI. The ELISA analytical biochemical technique of the MBS700914 kit is based on PI antibody-PI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PI elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS700914. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PI. It may not necessarily be applicable to this product.
 NCBI GI #    59036749
 NCBI GeneID    3630
 NCBI Accession #    AAW83741.1 [Other Products]
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 UniProt Primary Accession #    P01308 [Other Products]
 UniProt Secondary Accession #    Q5EEX2 [Other Products]
 UniProt Related Accession #    P01308 [Other Products]
 Molecular Weight    21,537 Da
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 NCBI Official Full Name    proinsulin
 NCBI Official Synonym Full Names    insulin
 NCBI Official Symbol    INS [Similar Products]
 NCBI Official Synonym Symbols   
ILPR; IRDN; IDDM1; IDDM2; MODY10
[Similar Products]
 NCBI Protein Information    insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2
 UniProt Protein Name    Insulin
 Protein Family    Floral homeotic protein
 UniProt Gene Name    INS [Similar Products]
 UniProt Entry Name    INS_HUMAN
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 NCBI Summary for PI    After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
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 UniProt Comments for PI    Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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 Research Articles on PI    1. Data suggest that plasma insulin levels can be modulated by dietary factors; here, plasma insulin levels are up-regulated by sugar-sweetened beverages (in men, increased consumption of 1 serving/day up-regulated plasma insulin).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PI elisa kitPathways associated with PI elisa kit
 Reference Product  PubMed Publications
 IRS1 elisa kit  >35 publications with PI and IRS1
 FOXO1 elisa kit  >31 publications with PI and FOXO1
 INSR elisa kit  >27 publications with PI and INSR
 IRS2 elisa kit  >26 publications with PI and IRS2
 AKT1 elisa kit  >18 publications with PI and AKT1
 GRB2 elisa kit  >11 publications with PI and GRB2
 IDE elisa kit  >7 publications with PI and IDE
 IGFBP1 elisa kit  >5 publications with PI and IGFBP1
 AKT2 elisa kit  >3 publications with PI and AKT2
 PTPN1 elisa kit  >3 publications with PI and PTPN1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
Diseases associated with PI elisa kitOrgans/Tissues associated with PI elisa kit
 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >1080 publications with PI and Metabolic Diseases
 Diabetes Mellitus Antibodies  >894 publications with PI and Diabetes Mellitus
 Insulin Resistance Antibodies  >326 publications with PI and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >307 publications with PI and Diabetes Mellitus, Type 2
 Obesity Antibodies  >241 publications with PI and Obesity
 Hyperglycemia Antibodies  >219 publications with PI and Hyperglycemia
 Hyperinsulinism Antibodies  >213 publications with PI and Hyperinsulinism
 Hypertension Antibodies  >129 publications with PI and Hypertension
 Polycystic Ovary Syndrome Antibodies  >59 publications with PI and Polycystic Ovary Syndrome
 Fatty Liver Antibodies  >48 publications with PI and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1326 publications with PI and Blood
 Pancreas Antibodies  >1266 publications with PI and Pancreas
 Brain Antibodies  >639 publications with PI and Brain
 Muscle Antibodies  >509 publications with PI and Muscle
 Liver Antibodies  >346 publications with PI and Liver
 Heart Antibodies  >263 publications with PI and Heart
 Vascular Antibodies  >242 publications with PI and Vascular
 Kidney Antibodies  >224 publications with PI and Kidney
 Nerve Antibodies  >219 publications with PI and Nerve
 Bone Antibodies  >140 publications with PI and Bone
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