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IL1A elisa kit :: Pig interleukin 1, alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703956
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interleukin 1, alpha (IL1A), ELISA Kit

★Popular Item★
 Also Known As   

Pig Interleukin 1alpha, IL-1alpha ELISA Kit

 Product Synonym Names    Pig Interleukin 1alpha; IL-1alpha ELISA kit; IL-1A; IL1; IL1-ALPHA; IL1F1; IL1A (IL1F1) ; OTTHUMP00000203692; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha; interleukin 1; alpha
 Product Gene Name   

IL1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703956 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P18430
 Species Reactivity    Pig
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Pig IL1A. No significant cross-reactivity or interference between Pig IL1A and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    25 pg/ml-1600 pg/ml
 Sensitivity    6.25pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 3    Inflammatory response
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL1A purchase    MBS703956 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 1, alpha (IL1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml-1600 pg/ml in biological research samples containing IL1A, with an estimated sensitivity of 6.25pg/ml. The ELISA analytical biochemical technique of the MBS703956 kit is based on IL1A antibody-IL1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL1A elisa kit

   For SampleFor the quantitative determination of pig interleukin 1? (IL-1?) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1? bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL1A elisa kit    IL1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703956. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL1A. It may not necessarily be applicable to this product.
 NCBI GI #    47522878
 NCBI GeneID    397094
 NCBI Accession #    NP_999194.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214029.1 [Other Products]
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 UniProt Primary Accession #    P18430 [Other Products]
 UniProt Related Accession #    P18430 [Other Products]
 Molecular Weight    30,789 Da
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 NCBI Official Full Name    interleukin-1 alpha
 NCBI Official Symbol    IL1A [Similar Products]
 NCBI Official Synonym Symbols   
IL-1alpha
[Similar Products]
 NCBI Protein Information    interleukin-1 alpha; IL-1 alpha; interleukin 1-alpha
 UniProt Protein Name    Interleukin-1 alpha
 Protein Family    Interleukin
 UniProt Gene Name    IL1A [Similar Products]
 UniProt Synonym Gene Names    IL-1 alpha [Similar Products]
 UniProt Entry Name    IL1A_PIG
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 UniProt Comments for IL1A    Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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 Research Articles on IL1A    1. Constitutive suppression of IL-1alpha maintains quiescence of endothelium and that terminal complement complexes remove that suppression, allowing IL-1alpha transcription and, ultimately, activation of endothelium to proceed.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL1A elisa kitPathways associated with IL1A elisa kit
 Reference Product  PubMed Publications
 IL6 elisa kit  >87 publications with IL1A and IL6
 IL-1beta elisa kit  >81 publications with IL1A and IL-1beta
 TNF elisa kit  >67 publications with IL1A and TNF
 IL1R1 elisa kit  >16 publications with IL1A and IL1R1
 NOS2 elisa kit  >5 publications with IL1A and NOS2
 IL1R2 elisa kit  >3 publications with IL1A and IL1R2
 MYD88 elisa kit  >3 publications with IL1A and MYD88
 TRAF6 elisa kit  >2 publications with IL1A and TRAF6
 NOD1 elisa kit  >1 publications with IL1A and NOD1
 IRAK4 elisa kit  >1 publications with IL1A and IRAK4
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with IL1A elisa kitOrgans/Tissues associated with IL1A elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >78 publications with IL1A and Inflammation
 Necrosis Antibodies  >56 publications with IL1A and Necrosis
 Skin Diseases Antibodies  >31 publications with IL1A and Skin Diseases
 Cardiovascular Diseases Antibodies  >24 publications with IL1A and Cardiovascular Diseases
 Kidney Diseases Antibodies  >8 publications with IL1A and Kidney Diseases
 Heart Diseases Antibodies  >7 publications with IL1A and Heart Diseases
 Liver Diseases Antibodies  >6 publications with IL1A and Liver Diseases
 Lung Neoplasms Antibodies  >6 publications with IL1A and Lung Neoplasms
 Pain Antibodies  >6 publications with IL1A and Pain
 Neoplasms, Experimental Antibodies  >5 publications with IL1A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >15 publications with IL1A and Skin
 Lung Antibodies  >10 publications with IL1A and Lung
 Vascular Antibodies  >10 publications with IL1A and Vascular
 Brain Antibodies  >8 publications with IL1A and Brain
 Testis Antibodies  >4 publications with IL1A and Testis
 Cervix Antibodies  >2 publications with IL1A and Cervix
 Lymph Node Antibodies  >2 publications with IL1A and Lymph Node
 Mouth Antibodies  >2 publications with IL1A and Mouth
 Intestine Antibodies  >1 publications with IL1A and Intestine
 Pharynx Antibodies  >1 publications with IL1A and Pharynx
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