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ALP elisa kit :: Porcine Alkaline phosphatase (ALP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267652
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alkaline phosphatase (ALP), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Alkaline phosphatase (ALP) ELISA Kit

 Product Gene Name   

ALP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    241510
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 Species Reactivity    Porcine
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 Specificity    No cross-reaction with other factors.
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 Samples    Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALP concentration
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78 ng/ml
 Sensitivity    0.08 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALP purchase    MBS267652 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline phosphatase (ALP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78 ng/ml in biological research samples containing ALP, with an estimated sensitivity of 0.08 ng/ml. The ELISA analytical biochemical technique of the MBS267652 kit is based on ALP antibody-ALP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ALP concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALP elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine ALP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ALP elisa kit    ALP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS267652. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALP. It may not necessarily be applicable to this product.
 NCBI GI #    178462
 NCBI GeneID    249
 NCBI Accession #    AAB59378.1 [Other Products]
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 UniProt Secondary Accession #    O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6 [Other Products]
 UniProt Related Accession #    P05186 [Other Products]
 Molecular Weight    51,045 Da
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 NCBI Official Full Name    alkaline phosphatase
 NCBI Official Synonym Full Names    alkaline phosphatase, liver/bone/kidney
 NCBI Official Symbol    ALPL [Similar Products]
 NCBI Official Synonym Symbols   
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
[Similar Products]
 NCBI Protein Information    alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
 UniProt Protein Name    Alkaline phosphatase, tissue-nonspecific isozyme
 UniProt Synonym Protein Names   
Alkaline phosphatase liver/bone/kidney isozyme
 Protein Family    Alkaline proteinase
 UniProt Gene Name    ALPL [Similar Products]
 UniProt Synonym Gene Names    AP-TNAP; TNSALP [Similar Products]
 UniProt Entry Name    PPBT_HUMAN
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 NCBI Summary for ALP    There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
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 UniProt Comments for ALP    ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; EC 3.1.3.1; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular matrix; extracellular space; membrane; integral to membrane; plasma membrane

Molecular Function: pyrophosphatase activity; protein binding; alkaline phosphatase activity; metal ion binding

Biological Process: osteoblast differentiation; response to antibiotic; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification

Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood
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 Research Articles on ALP    1. ABO locus is a major determinant for serum ALP levels in Chinese Han population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ALP elisa kitDiseases associated with ALP elisa kit
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 Folate Biosynthesis Pathway antibodies  Folate Biosynthesis Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with ALP and Neoplasms
 Kidney Diseases Antibodies  >9 publications with ALP and Kidney Diseases
 Hypophosphatasia, Infantile Antibodies  >9 publications with ALP and Hypophosphatasia, Infantile
 Hypophosphatasia, Childhood Antibodies  >8 publications with ALP and Hypophosphatasia, Childhood
 Cardiovascular Diseases Antibodies  >8 publications with ALP and Cardiovascular Diseases
 Seizures Antibodies  >7 publications with ALP and Seizures
 Inflammation Antibodies  >6 publications with ALP and Inflammation
 Pain Antibodies  >6 publications with ALP and Pain
 Calcinosis Antibodies  >5 publications with ALP and Calcinosis
 Hypertension Antibodies  >4 publications with ALP and Hypertension
Organs/Tissues associated with ALP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >129 publications with ALP and Bone
 Blood Antibodies  >43 publications with ALP and Blood
 Liver Antibodies  >35 publications with ALP and Liver
 Kidney Antibodies  >35 publications with ALP and Kidney
 Connective Tissue Antibodies  >29 publications with ALP and Connective Tissue
 Brain Antibodies  >12 publications with ALP and Brain
 Mouth Antibodies  >11 publications with ALP and Mouth
 Adipose Tissue Antibodies  >10 publications with ALP and Adipose Tissue
 Embryonic Tissue Antibodies  >6 publications with ALP and Embryonic Tissue
 Skin Antibodies  >6 publications with ALP and Skin
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