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AT2R1 elisa kit :: Porcine Angiotensin 2 Receptor 1 Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS743547
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin 2 Receptor 1 Antibody (AT2R1), ELISA Kit

 Also Known As   

Porcine Angiotensin 2 Receptor 1 Antibody ELISA Kit

 Product Gene Name   

AT2R1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 267430
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 Species Reactivity    Porcine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This AT1R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AT1R-AA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AT2R1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AT2R1 purchase    MBS743547 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin 2 Receptor 1 Antibody (AT2R1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AT2R1, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS743547 kit is based on AT2R1 antibody-AT2R1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT2R1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT2R1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AT2R1 elisa kit

   Principle of the Assay: AT1R-AA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT1R-AA antibody and an AT1R-AA-HRP conjugate. The assay sample and buffer are incubated together with AT1R-AA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT1R-AA concentration since AT1R-AA from samples and AT1R-AA-HRP conjugate compete for the anti-AT1R-AA antibody binding site. Since the number of sites is limited, as more sites are occupied by AT1R-AA from the sample, fewer sites are left to bind AT1R-AA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AT2R1 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of AT2R1 elisa kit    AT2R1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS743547. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AT2R1. It may not necessarily be applicable to this product.
 NCBI GI #    46250427
 NCBI GeneID    185
 NCBI Accession #    AAH68494.1 [Other Products]
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 UniProt Secondary Accession #    Q13725; Q8TBK4 [Other Products]
 UniProt Related Accession #    P30556 [Other Products]
 Molecular Weight    41,061 Da
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 NCBI Official Full Name    Angiotensin II receptor, type 1
 NCBI Official Synonym Full Names    angiotensin II receptor, type 1
 NCBI Official Symbol    AGTR1 [Similar Products]
 NCBI Official Synonym Symbols   
AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B
[Similar Products]
 NCBI Protein Information    type-1 angiotensin II receptor; type-1B angiotensin II receptor
 UniProt Protein Name    Type-1 angiotensin II receptor
 UniProt Synonym Protein Names   
AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1
 UniProt Gene Name    AGTR1 [Similar Products]
 UniProt Synonym Gene Names    AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1 [Similar Products]
 UniProt Entry Name    AGTR1_HUMAN
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 NCBI Summary for AT2R1    Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]
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 UniProt Comments for AT2R1    AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; angiotensin type II receptor activity; protein heterodimerization activity; angiotensin type I receptor activity; bradykinin receptor binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of vasodilation; positive regulation of cellular protein metabolic process; renin-angiotensin regulation of aldosterone production; calcium-mediated signaling; regulation of systemic arterial blood pressure by renin-angiotensin; Rho protein signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; renin-angiotensin regulation of blood vessel size; regulation of vasoconstriction; regulation of inflammatory response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of cell growth; kidney development; positive regulation of phospholipase A2 activity; positive regulation of NAD(P)H oxidase activity; positive regulation of inflammatory response

Disease: Renal Tubular Dysgenesis; Hypertension, Essential
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 Research Articles on AT2R1    1. AT1 A1166C independently and in combination with MMP9 R668Q and NFKB1-94 ATTG ins/del polymorphisms plays important role in conferring genetic susceptibility to LVD in CAD patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AT2R1 elisa kitPathways associated with AT2R1 elisa kit
 Reference Product  PubMed Publications
 AGT elisa kit  >109 publications with AT2R1 and AGT
 NOS3 elisa kit  >18 publications with AT2R1 and NOS3
 BDKRB2 elisa kit  >9 publications with AT2R1 and BDKRB2
 EDN1 elisa kit  >5 publications with AT2R1 and EDN1
 KNG1 elisa kit  >1 publications with AT2R1 and KNG1
 GRK5 elisa kit  >1 publications with AT2R1 and GRK5
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
Diseases associated with AT2R1 elisa kitOrgans/Tissues associated with AT2R1 elisa kit
 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >115 publications with AT2R1 and Vascular Diseases
 Hypertension Antibodies  >108 publications with AT2R1 and Hypertension
 Heart Diseases Antibodies  >68 publications with AT2R1 and Heart Diseases
 Kidney Diseases Antibodies  >37 publications with AT2R1 and Kidney Diseases
 Atherosclerosis Antibodies  >25 publications with AT2R1 and Atherosclerosis
 Hypertrophy Antibodies  >20 publications with AT2R1 and Hypertrophy
 Inflammation Antibodies  >20 publications with AT2R1 and Inflammation
 Diabetic Nephropathies Antibodies  >16 publications with AT2R1 and Diabetic Nephropathies
 Hypertrophy, Left Ventricular Antibodies  >15 publications with AT2R1 and Hypertrophy, Left Ventricular
 Heart Failure Antibodies  >14 publications with AT2R1 and Heart Failure
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >52 publications with AT2R1 and Heart
 Kidney Antibodies  >37 publications with AT2R1 and Kidney
 Muscle Antibodies  >27 publications with AT2R1 and Muscle
 Brain Antibodies  >15 publications with AT2R1 and Brain
 Lung Antibodies  >11 publications with AT2R1 and Lung
 Placenta Antibodies  >11 publications with AT2R1 and Placenta
 Liver Antibodies  >9 publications with AT2R1 and Liver
 Connective Tissue Antibodies  >8 publications with AT2R1 and Connective Tissue
 Embryonic Tissue Antibodies  >7 publications with AT2R1 and Embryonic Tissue
 Intestine Antibodies  >4 publications with AT2R1 and Intestine
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