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AP-12 elisa kit :: Porcine Apelin-12 (AP-12) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS261347
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
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 Product Name   

Apelin-12 (AP-12), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Apelin-12 (AP-12) ELISA Kit

 Product Gene Name   

AP-12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600052
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 Species Reactivity    Porcine
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 Specificity    No cross-reaction with other factors.
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 Samples    Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant AP-12 concentration.
 Assay Type    Sandwich
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    12 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AP-12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AP-12 purchase    MBS261347 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apelin-12 (AP-12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing AP-12, with an estimated sensitivity of 12 pg/ml. The ELISA analytical biochemical technique of the MBS261347 kit is based on AP-12 antibody-AP-12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP-12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP-12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant AP-12 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AP-12 elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine AP-12 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve of AP-12 elisa kit    AP-12 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS261347. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AP-12. It may not necessarily be applicable to this product.
 NCBI GI #    4885057
 NCBI GeneID    187
 NCBI Accession #    NP_005152.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005161.4 [Other Products]
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 UniProt Related Accession #    P35414 [Other Products]
 Molecular Weight    42,660 Da
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 NCBI Official Full Name    apelin receptor
 NCBI Official Synonym Full Names    apelin receptor
 NCBI Official Symbol    APLNR [Similar Products]
 NCBI Official Synonym Symbols   
APJ; APJR; HG11; AGTRL1
[Similar Products]
 NCBI Protein Information    apelin receptor; APJ receptor; HG11 orphan receptor; APJ (apelin) receptor; angiotensin receptor-like 1; G protein-coupled receptor APJ; G-protein coupled receptor APJ; angiotensin II receptor-like 1; G-protein coupled receptor HG11
 UniProt Protein Name    Apelin receptor
 UniProt Synonym Protein Names   
Angiotensin receptor-like 1; G-protein coupled receptor APJ; G-protein coupled receptor HG11
 UniProt Gene Name    APLNR [Similar Products]
 UniProt Synonym Gene Names    AGTRL1; APJ [Similar Products]
 UniProt Entry Name    APJ_HUMAN
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 NCBI Summary for AP-12    This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]
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 UniProt Comments for AP-12    AGTRL1: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of body fluid levels; heart development; gastrulation
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 Research Articles on AP-12    1. functional role of the apelin--APJ system likely in early gestation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AP-12 elisa kitPathways associated with AP-12 elisa kit
 Reference Product  PubMed Publications
 APLN elisa kit  >163 publications with AP-12 and APLN
 VEGFA elisa kit  >2 publications with AP-12 and VEGFA
 POMC elisa kit  >2 publications with AP-12 and POMC
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with AP-12 elisa kitOrgans/Tissues associated with AP-12 elisa kit
 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >51 publications with AP-12 and Vascular Diseases
 Heart Diseases Antibodies  >35 publications with AP-12 and Heart Diseases
 Hypertension Antibodies  >33 publications with AP-12 and Hypertension
 Disease Models, Animal Antibodies  >19 publications with AP-12 and Disease Models, Animal
 Nervous System Diseases Antibodies  >13 publications with AP-12 and Nervous System Diseases
 Hypertrophy Antibodies  >11 publications with AP-12 and Hypertrophy
 Inflammation Antibodies  >11 publications with AP-12 and Inflammation
 Kidney Diseases Antibodies  >8 publications with AP-12 and Kidney Diseases
 Brain Diseases Antibodies  >8 publications with AP-12 and Brain Diseases
 Lung Diseases Antibodies  >8 publications with AP-12 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >91 publications with AP-12 and Blood
 Heart Antibodies  >67 publications with AP-12 and Heart
 Brain Antibodies  >40 publications with AP-12 and Brain
 Kidney Antibodies  >19 publications with AP-12 and Kidney
 Lung Antibodies  >18 publications with AP-12 and Lung
 Embryonic Tissue Antibodies  >15 publications with AP-12 and Embryonic Tissue
 Connective Tissue Antibodies  >13 publications with AP-12 and Connective Tissue
 Adipose Tissue Antibodies  >12 publications with AP-12 and Adipose Tissue
 Eye Antibodies  >8 publications with AP-12 and Eye
 Ovary Antibodies  >6 publications with AP-12 and Ovary
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