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CYP7A1 elisa kit :: Porcine CYP7A1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2513085 CYP7A1 elisa kit
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CYP7A1, ELISA Kit

 Also Known As   

Porcine CYP7A1 (Cytochrome P450 7A1) ELISA Kit

 Product Gene Name   

CYP7A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    118455
 3D Structure    ModBase 3D Structure for P22680
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 Species Reactivity    Porcine
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 Specificity    This kit recognizes natural and recombinant Porcine CYP7A1. No significant cross-reactivity or interference between Porcine CYP7A1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.781-50ng/mL
 Sensitivity    0.469ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP7A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP7A1 purchase    MBS2513085 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CYP7A1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing CYP7A1, with an estimated sensitivity of 0.469ng/mL. The ELISA analytical biochemical technique of the MBS2513085 kit is based on CYP7A1 antibody-CYP7A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP7A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP7A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CYP7A1 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine CYP7A1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYP7A1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYP7A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYP7A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYP7A1. You can calculate the concentration of CYP7A1 in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2513085. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CYP7A1. It may not necessarily be applicable to this product.
 NCBI GI #    166295200
 NCBI GeneID    1581
 NCBI Accession #    NP_000771.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000780.3 [Other Products]
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 UniProt Primary Accession #    P22680 [Other Products]
 UniProt Secondary Accession #    P78454; Q3MIL8; Q7KZ19 [Other Products]
 UniProt Related Accession #    P22680 [Other Products]
 Molecular Weight    57,661 Da
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 NCBI Official Full Name    cholesterol 7-alpha-monooxygenase
 NCBI Official Synonym Full Names    cytochrome P450, family 7, subfamily A, polypeptide 1
 NCBI Official Symbol    CYP7A1 [Similar Products]
 NCBI Official Synonym Symbols   
CP7A; CYP7; CYPVII
[Similar Products]
 NCBI Protein Information    cholesterol 7-alpha-monooxygenase; cholesterol 7-alpha-hydroxylase; cytochrome P450 7A1; cytochrome P450, subfamily VIIA polypeptide 1
 UniProt Protein Name    Cholesterol 7-alpha-monooxygenase
 UniProt Synonym Protein Names   
CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1
 Protein Family    Cholesterol 7-alpha-monooxygenase
 UniProt Gene Name    CYP7A1 [Similar Products]
 UniProt Synonym Gene Names    CYP7 [Similar Products]
 UniProt Entry Name    CP7A1_HUMAN
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 NCBI Summary for CYP7A1    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]
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 UniProt Comments for CYP7A1    CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.17; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q11-q12

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: cholesterol 7-alpha-monooxygenase activity; iron ion binding; heme binding

Biological Process: bile acid biosynthetic process; cholesterol homeostasis; bile acid metabolic process; xenobiotic metabolic process; cellular lipid metabolic process; cholesterol catabolic process; transmembrane transport; sterol metabolic process
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 Research Articles on CYP7A1    1. Cyp7a1 is a direct Thyroid hormone receptor (TR) target gene that responds to physiologic TR levels through a set of distinct response elements in its promoter.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CYP7A1 elisa kitPathways associated with CYP7A1 elisa kit
 Reference Product  PubMed Publications
 CYP27A1 elisa kit  >86 publications with CYP7A1 and CYP27A1
 NR5A2 elisa kit  >21 publications with CYP7A1 and NR5A2
 NR0B2 elisa kit  >9 publications with CYP7A1 and NR0B2
 NR1H4 elisa kit  >5 publications with CYP7A1 and NR1H4
 PPARA elisa kit  >4 publications with CYP7A1 and PPARA
 POR elisa kit  >2 publications with CYP7A1 and POR
 HSD3B2 elisa kit  >2 publications with CYP7A1 and HSD3B2
 HSD3B7 elisa kit  >2 publications with CYP7A1 and HSD3B7
 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway antibodies  Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway Diagram
 Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway antibodies  Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway Diagram
 Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway antibodies  Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway Diagram
Diseases associated with CYP7A1 elisa kitOrgans/Tissues associated with CYP7A1 elisa kit
 Disease Name  Pubmed Publications
 Hypercholesterolemia Antibodies  >205 publications with CYP7A1 and Hypercholesterolemia
 Fatty Liver Antibodies  >200 publications with CYP7A1 and Fatty Liver
 Dyslipidemias Antibodies  >194 publications with CYP7A1 and Dyslipidemias
 Liver Diseases Antibodies  >190 publications with CYP7A1 and Liver Diseases
 Hyperlipidemias Antibodies  >184 publications with CYP7A1 and Hyperlipidemias
 Atherosclerosis Antibodies  >139 publications with CYP7A1 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >95 publications with CYP7A1 and Cardiovascular Diseases
 Cholestasis Antibodies  >95 publications with CYP7A1 and Cholestasis
 Liver Neoplasms Antibodies  >56 publications with CYP7A1 and Liver Neoplasms
 Inflammation Antibodies  >51 publications with CYP7A1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1458 publications with CYP7A1 and Liver
 Testis Antibodies  >9 publications with CYP7A1 and Testis
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