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CLPS elisa kit :: Porcine Colipase (CLPS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7217980
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Colipase (CLPS), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Colipase (CLPS) ELISA Kit

 Product Gene Name   

CLPS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    120105
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 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CLPS. No significant cross-reactivity or interference between CLPS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLPS and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLPS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLPS purchase    MBS7217980 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Colipase (CLPS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLPS, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS7217980 kit is based on CLPS antibody-CLPS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLPS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLPS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLPS elisa kit

   Intended Uses: This CLPS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CLPS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CLPS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLPS antibody and an CLPS-HRP conjugate. The assay sample and buffer are incubated together with CLPS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLPS concentration since CLPS from samples and CLPS-HRP conjugate compete for the anti-CLPS antibody binding site. Since the number of sites is limited, as more sites are occupied by CLPS from the sample, fewer sites are left to bind CLPS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLPS concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of CLPS elisa kit    CLPS elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7217980. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLPS. It may not necessarily be applicable to this product.
 NCBI GI #    180886
 NCBI GeneID    1208
 NCBI Accession #    AAA52054.1 [Other Products]
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 UniProt Secondary Accession #    Q5T9G7; Q5U809 [Other Products]
 UniProt Related Accession #    P04118 [Other Products]
 Molecular Weight    11,954 Da
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 NCBI Official Full Name    colipase
 NCBI Official Synonym Full Names    colipase, pancreatic
 NCBI Official Symbol    CLPS [Similar Products]
 NCBI Protein Information    colipase; pancreatic colipase preproprotein
 UniProt Protein Name    Colipase
 Protein Family    Colipase
 UniProt Gene Name    CLPS [Similar Products]
 UniProt Entry Name    COL_HUMAN
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 NCBI Summary for CLPS    The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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 UniProt Comments for CLPS    CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family.

Protein type: Secreted, signal peptide; Activator; Secreted

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: extracellular region

Molecular Function: enzyme activator activity

Biological Process: positive regulation of catalytic activity; phototransduction, visible light; lipid metabolic process; retinoid metabolic process; lipid catabolic process; lipid digestion
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 Research Articles on CLPS    1. Low CLPS expression is associated with pancreatic cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CLPS elisa kitPathways associated with CLPS elisa kit
 Reference Product  PubMed Publications
 GMPS elisa kit  >7 publications with CLPS and GMPS
 LY6D elisa kit  >7 publications with CLPS and LY6D
 PNLIP elisa kit  >3 publications with CLPS and PNLIP
 ARL4C elisa kit  >1 publications with CLPS and ARL4C
 JDP2 elisa kit  >1 publications with CLPS and JDP2
 PNLIPRP1 elisa kit  >1 publications with CLPS and PNLIPRP1
 PNLIPRP2 elisa kit  >1 publications with CLPS and PNLIPRP2
 CEL elisa kit  >1 publications with CLPS and CEL
 Products by Pathway  Pathway Diagram
 Digestion Of Dietary Lipid Pathway antibodies  Digestion Of Dietary Lipid Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Retinoid Metabolism And Transport Pathway antibodies  Retinoid Metabolism And Transport Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with CLPS elisa kitOrgans/Tissues associated with CLPS elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >15 publications with CLPS and Cardiovascular Diseases
 Inflammation Antibodies  >13 publications with CLPS and Inflammation
 Atherosclerosis Antibodies  >9 publications with CLPS and Atherosclerosis
 Immune System Diseases Antibodies  >7 publications with CLPS and Immune System Diseases
 Carcinoma Antibodies  >7 publications with CLPS and Carcinoma
 Genital Diseases, Male Antibodies  >6 publications with CLPS and Genital Diseases, Male
 Diabetes Mellitus Antibodies  >4 publications with CLPS and Diabetes Mellitus
 Breast Neoplasms Antibodies  >3 publications with CLPS and Breast Neoplasms
 Necrosis Antibodies  >3 publications with CLPS and Necrosis
 Liver Diseases Antibodies  >3 publications with CLPS and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >109 publications with CLPS and Blood
 Spleen Antibodies  >16 publications with CLPS and Spleen
 Pancreas Antibodies  >11 publications with CLPS and Pancreas
 Liver Antibodies  >10 publications with CLPS and Liver
 Brain Antibodies  >4 publications with CLPS and Brain
 Ovary Antibodies  >1 publications with CLPS and Ovary
 Testis Antibodies  >1 publications with CLPS and Testis
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