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C5A elisa kit :: Porcine Complement Fragment 5A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS027625 C5A elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Fragment 5A (C5A), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Complement Fragment 5A ELISA Kit

 Product Gene Name   

C5A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609949
 3D Structure    ModBase 3D Structure for Q9P296
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 Species Reactivity    Porcine
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 Specificity    No significant cross-reactivity or interference between Porcine C5A and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    6.25 ng/ml - 200 ng/ml.
 Sensitivity    1.0 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C5A concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine C5A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C5A and C5A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C5A purchase    MBS027625 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Fragment 5A (C5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 ng/ml - 200 ng/ml in biological research samples containing C5A, with an estimated sensitivity of 1.0 ng/ml. The ELISA analytical biochemical technique of the MBS027625 kit is based on C5A antibody-C5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C5A elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C5A concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine C5A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C5A and C5A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS027625. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C5A. It may not necessarily be applicable to this product.
 NCBI GI #    8923873
 NCBI GeneID    27202
 NCBI Accession #    NP_060955.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018485.2 [Other Products]
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 UniProt Primary Accession #    Q9P296 [Other Products]
 UniProt Secondary Accession #    B2RA09 [Other Products]
 UniProt Related Accession #    Q9P296 [Other Products]
 Molecular Weight    36,080 Da
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 NCBI Official Full Name    C5a anaphylatoxin chemotactic receptor 2
 NCBI Official Synonym Full Names    complement component 5a receptor 2
 NCBI Official Symbol    C5AR2 [Similar Products]
 NCBI Official Synonym Symbols   
C5L2; GPF77; GPR77
[Similar Products]
 NCBI Protein Information    C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2
 UniProt Protein Name    C5a anaphylatoxin chemotactic receptor 2
 UniProt Synonym Protein Names   
Complement component 5a receptor 2; G-protein coupled receptor 77
 UniProt Gene Name    C5AR2 [Similar Products]
 UniProt Synonym Gene Names    C5L2; GPR77 [Similar Products]
 UniProt Entry Name    C5AR2_HUMAN
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 NCBI Summary for C5A    This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
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 UniProt Comments for C5A    C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: apical part of cell; plasma membrane; integral to membrane; basal plasma membrane

Molecular Function: protein binding; C5a anaphylatoxin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of tumor necrosis factor production; chemotaxis; inflammatory response; positive regulation of epithelial cell proliferation
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 Research Articles on C5A    1. A novel polymorphism (901G > a) of C5L2 gene is associated with coronary artery disease in Chinese Han and Uyghur population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C5A elisa kitPathways associated with C5A elisa kit
 Reference Product  PubMed Publications
 C5AR1 elisa kit  >1 publications with C5A and C5AR1
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Organs/Tissues associated with C5A elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with C5A and Brain
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