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DHT elisa kit :: Porcine Dihydrotestosterone (DHT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266158
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dihydrotestosterone (DHT), ELISA Kit

 Also Known As   

Porcine Dihydrotestosterone (DHT) ELISA Kit

 Product Gene Name   

DHT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Porcine
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 Specificity    No cross-reaction with other factors.
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 Samples    Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DHT concentration
 Assay Type    Sandwich
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    12 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DHT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DHT purchase    MBS266158 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydrotestosterone (DHT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing DHT, with an estimated sensitivity of 12 pg/ml. The ELISA analytical biochemical technique of the MBS266158 kit is based on DHT antibody-DHT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DHT concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DHT elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine DHT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of DHT elisa kit    DHT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266158. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DHT. It may not necessarily be applicable to this product.
 NCBI GI #    1903379
 NCBI Accession #    AAB58249.1 [Other Products]
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 UniProt Secondary Accession #    P97862 [Other Products]
 Molecular Weight    43,009 Da
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 NCBI Official Full Name    differentially repressed by testosterone and dihydrotestosterone
 UniProt Protein Name    Protein NDRG1
 UniProt Synonym Protein Names   
N-myc downstream-regulated gene 1 protein; Protein Ndr1
 Protein Family    D-hydantoinase/dihydropyrimidinase
 UniProt Gene Name    Ndrg1 [Similar Products]
 UniProt Synonym Gene Names    Ndr1; Ndrl; Tdd5; Protein Ndr1 [Similar Products]
 UniProt Entry Name    NDRG1_MOUSE
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 UniProt Comments for DHT    NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).

Protein type: Cell development/differentiation; Vesicle

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cytoskeleton; cell-cell adherens junction; membrane; perinuclear region of cytoplasm; recycling endosome membrane; cytoplasm; plasma membrane; nucleus; myelin sheath

Molecular Function: gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: myelin maintenance in the peripheral nervous system; mast cell activation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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