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IL-1alpha elisa kit :: Porcine IL-1alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510794 IL-1alpha elisa kit
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,975 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,480 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-1alpha, ELISA Kit

★Popular Item★
 Also Known As   

Porcine IL-1alpha(Interleukin 1 Alpha)ELISA Kit

 Product Gene Name   

IL-1alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2510794 COA    COA PDF
 OMIM    147760
 3D Structure    ModBase 3D Structure for P01583
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 Species Reactivity    Porcine
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 Specificity    This kit recognizes natural and some recombinant Porcine IL-1?. No Significant cross -reactivity or interference between Porcine IL-1? and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-1alpha purchase    MBS2510794 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-1alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing IL-1alpha, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2510794 kit is based on IL-1alpha antibody-IL-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-1alpha elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-1alpha in Porcine serum, plasma and other biological fluids

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine IL-1alpha. You can calculate the concentration of Porcine IL-1alpha in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2510794. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-1alpha. It may not necessarily be applicable to this product.
 NCBI GI #    27894330
 NCBI GeneID    3552
 NCBI Accession #    NP_000566.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000575.3 [Other Products]
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 UniProt Primary Accession #    P01583 [Other Products]
 UniProt Secondary Accession #    Q53QF9; Q7RU02 [Other Products]
 UniProt Related Accession #    P01583 [Other Products]
 Molecular Weight    30,607 Da
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 NCBI Official Full Name    interleukin-1 alpha proprotein
 NCBI Official Synonym Full Names    interleukin 1, alpha
 NCBI Official Symbol    IL1A [Similar Products]
 NCBI Official Synonym Symbols   
IL1; IL-1A; IL1F1; IL1-ALPHA
[Similar Products]
 NCBI Protein Information    interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
 UniProt Protein Name    Interleukin-1 alpha
 UniProt Synonym Protein Names   
Hematopoietin-1
 UniProt Gene Name    IL1A [Similar Products]
 UniProt Synonym Gene Names    IL1F1; IL-1 alpha [Similar Products]
 UniProt Entry Name    IL1A_HUMAN
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 NCBI Summary for IL-1alpha    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-1alpha    IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of cytokine secretion
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 Research Articles on IL-1alpha    1. Elevated serum IL1a was associated with increased ovarian cancer risk.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-1alpha elisa kitPathways associated with IL-1alpha elisa kit
 Reference Product  PubMed Publications
 IL1B elisa kit  >195 publications with IL-1alpha and IL1B
 IL6 elisa kit  >94 publications with IL-1alpha and IL6
 IL8 elisa kit  >39 publications with IL-1alpha and IL8
 IL1R1 elisa kit  >16 publications with IL-1alpha and IL1R1
 CSF2 elisa kit  >10 publications with IL-1alpha and CSF2
 MYD88 elisa kit  >4 publications with IL-1alpha and MYD88
 IL1R2 elisa kit  >3 publications with IL-1alpha and IL1R2
 TRAF6 elisa kit  >2 publications with IL-1alpha and TRAF6
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Graft-versus-host Disease Pathway antibodies  Graft-versus-host Disease Pathway Diagram
Diseases associated with IL-1alpha elisa kitOrgans/Tissues associated with IL-1alpha elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >85 publications with IL-1alpha and Inflammation
 Necrosis Antibodies  >59 publications with IL-1alpha and Necrosis
 Skin Diseases Antibodies  >31 publications with IL-1alpha and Skin Diseases
 Pain Antibodies  >8 publications with IL-1alpha and Pain
 Heart Diseases Antibodies  >8 publications with IL-1alpha and Heart Diseases
 Kidney Diseases Antibodies  >8 publications with IL-1alpha and Kidney Diseases
 Lung Neoplasms Antibodies  >7 publications with IL-1alpha and Lung Neoplasms
 Fibrosis Antibodies  >6 publications with IL-1alpha and Fibrosis
 Liver Diseases Antibodies  >6 publications with IL-1alpha and Liver Diseases
 Hypertension Antibodies  >5 publications with IL-1alpha and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >22 publications with IL-1alpha and Skin
 Lung Antibodies  >20 publications with IL-1alpha and Lung
 Vascular Antibodies  >18 publications with IL-1alpha and Vascular
 Brain Antibodies  >13 publications with IL-1alpha and Brain
 Mouth Antibodies  >5 publications with IL-1alpha and Mouth
 Testis Antibodies  >4 publications with IL-1alpha and Testis
 Intestine Antibodies  >3 publications with IL-1alpha and Intestine
 Cervix Antibodies  >2 publications with IL-1alpha and Cervix
 Lymph Node Antibodies  >2 publications with IL-1alpha and Lymph Node
 Pharynx Antibodies  >1 publications with IL-1alpha and Pharynx
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