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IGF-1 elisa kit :: Porcine Insulin Like Growth Factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761388
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Insulin Like Growth Factor 1 (IGF-1), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Insulin Like Growth Factor 1 ELISA Kit

 Product Gene Name   

IGF-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS761388 COA    COA PDF
 MBS761388 QC Report    QC Report PDF
 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IGF-1. No significant cross-reactivity or interference between IGF-1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.313-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF- 1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF- 1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IGF-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IGF-1 purchase    MBS761388 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Like Growth Factor 1 (IGF-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing IGF-1, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS761388 kit is based on IGF-1 antibody-IGF-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGF-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGF-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IGF-1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGF-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IGF-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGF-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGF-1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of IGF-1 elisa kit    IGF-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS761388. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IGF-1. It may not necessarily be applicable to this product.
 NCBI GI #    118150782
 NCBI GeneID    281239
 NCBI Accession #    NP_001071296.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001077828.1 [Other Products]
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 UniProt Related Accession #    P07455 [Other Products]
 Molecular Weight    17,066 Da
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 NCBI Official Full Name    insulin-like growth factor I preproprotein
 NCBI Official Symbol    IGF1 [Similar Products]
 NCBI Official Synonym Symbols   
IGF-1; IGF-I
[Similar Products]
 NCBI Protein Information    insulin-like growth factor I
 UniProt Protein Name    Insulin-like growth factor I
 UniProt Synonym Protein Names   
Somatomedin
 UniProt Gene Name    IGF1 [Similar Products]
 UniProt Synonym Gene Names    IGF-I [Similar Products]
 UniProt Entry Name    IGF1_BOVIN
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 UniProt Comments for IGF-1    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().
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 Research Articles on IGF-1    1. These findings strongly support the concept that IGF-1 upregulates LHR expression in granulosa cells and that IGF-1 is required for determining which follicle becomes dominant and acquires ovulatory capacity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IGF-1 elisa kitDiseases associated with IGF-1 elisa kit
 Reference Product  PubMed Publications
 IGF1R elisa kit  >515 publications with IGF-1 and IGF1R
 IGFBP3 elisa kit  >251 publications with IGF-1 and IGFBP3
 IGFBP1 elisa kit  >91 publications with IGF-1 and IGFBP1
 IRS1 elisa kit  >74 publications with IGF-1 and IRS1
 IGFBP2 elisa kit  >71 publications with IGF-1 and IGFBP2
 INSR elisa kit  >48 publications with IGF-1 and INSR
 IGFBP5 elisa kit  >34 publications with IGF-1 and IGFBP5
 IGFBP4 elisa kit  >29 publications with IGF-1 and IGFBP4
 SHC1 elisa kit  >1 publications with IGF-1 and SHC1
 Disease Name  Pubmed Publications
 Acromegaly Antibodies  >149 publications with IGF-1 and Acromegaly
 Breast Neoplasms Antibodies  >128 publications with IGF-1 and Breast Neoplasms
 Inflammation Antibodies  >105 publications with IGF-1 and Inflammation
 Female Urogenital Diseases Antibodies  >97 publications with IGF-1 and Female Urogenital Diseases
 Adenoma Antibodies  >78 publications with IGF-1 and Adenoma
 Liver Diseases Antibodies  >77 publications with IGF-1 and Liver Diseases
 Prostatic Neoplasms Antibodies  >58 publications with IGF-1 and Prostatic Neoplasms
 Necrosis Antibodies  >58 publications with IGF-1 and Necrosis
 Pituitary Neoplasms Antibodies  >52 publications with IGF-1 and Pituitary Neoplasms
 Hypertension Antibodies  >42 publications with IGF-1 and Hypertension
Organs/Tissues associated with IGF-1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >337 publications with IGF-1 and Muscle
 Lung Antibodies  >99 publications with IGF-1 and Lung
 Skin Antibodies  >50 publications with IGF-1 and Skin
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