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ICAM1 elisa kit :: Porcine Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023493 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $525 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,255 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Intercellular Adhesion Molecule 1 (ICAM1), ELISA Kit

★Popular Item★
 Also Known As   

Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit

 Product Synonym Names    CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
 Product Gene Name   

ICAM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147840
 3D Structure    ModBase 3D Structure for P05362
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 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1).
No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    6.25-100ng/mL
 Sensitivity    < 2.67ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ICAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ICAM1 purchase    MBS2023493 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25-100ng/mL in biological research samples containing ICAM1, with an estimated sensitivity of < 2.67ng/mL. The ELISA analytical biochemical technique of the MBS2023493 kit is based on ICAM1 antibody-ICAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ICAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ICAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ICAM1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 1 (ICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 1 (ICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ICAM1 elisa kit    CD & Adhesion molecule; Tumor immunity; Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of ICAM1 elisa kit    ICAM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023493. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ICAM1. It may not necessarily be applicable to this product.
 NCBI GI #    167466198
 NCBI GeneID    3383
 NCBI Accession #    NP_000192.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000201.2 [Other Products]
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 UniProt Primary Accession #    P05362 [Other Products]
 UniProt Secondary Accession #    Q5NKV7; Q96B50; B2R6M3 [Other Products]
 UniProt Related Accession #    P05362 [Other Products]
 Molecular Weight    57,825 Da
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 NCBI Official Full Name    intercellular adhesion molecule 1
 NCBI Official Synonym Full Names    intercellular adhesion molecule 1
 NCBI Official Symbol    ICAM1 [Similar Products]
 NCBI Official Synonym Symbols   
BB2; CD54; P3.58
[Similar Products]
 NCBI Protein Information    intercellular adhesion molecule 1
 UniProt Protein Name    Intercellular adhesion molecule 1
 UniProt Synonym Protein Names   
Major group rhinovirus receptor; CD_antigen: CD54
 Protein Family    Intercellular adhesion molecule
 UniProt Gene Name    ICAM1 [Similar Products]
 UniProt Synonym Gene Names    ICAM-1 [Similar Products]
 UniProt Entry Name    ICAM1_HUMAN
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 NCBI Summary for ICAM1    This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ICAM1    ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: integrin binding; viral receptor activity; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; cellular response to nutrient levels; leukocyte adhesion; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To
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 Research Articles on ICAM1    1. Levels of ICAM-1 and TIMP-1 were significantly elevated in set 1 in PDAC patients with jaundice compared to PDAC patients without jaundice and both proteins were elevated in patients with jaundice due to gall stones.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ICAM1 elisa kitPathways associated with ICAM1 elisa kit
 Reference Product  PubMed Publications
 ITGB2 elisa kit  >880 publications with ICAM1 and ITGB2
 ITGAL elisa kit  >474 publications with ICAM1 and ITGAL
 ITGAM elisa kit  >440 publications with ICAM1 and ITGAM
 CD44 elisa kit  >371 publications with ICAM1 and CD44
 IL6 elisa kit  >152 publications with ICAM1 and IL6
 ITGAX elisa kit  >139 publications with ICAM1 and ITGAX
 SELE elisa kit  >86 publications with ICAM1 and SELE
 HLA-B elisa kit  >29 publications with ICAM1 and HLA-B
 DIF elisa kit  >3 publications with ICAM1 and DIF
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
Diseases associated with ICAM1 elisa kitOrgans/Tissues associated with ICAM1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5612 publications with ICAM1 and Inflammation
 Necrosis Antibodies  >4779 publications with ICAM1 and Necrosis
 Cardiovascular Diseases Antibodies  >3643 publications with ICAM1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1463 publications with ICAM1 and Atherosclerosis
 Reperfusion Injury Antibodies  >880 publications with ICAM1 and Reperfusion Injury
 Myocardial Ischemia Antibodies  >794 publications with ICAM1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >759 publications with ICAM1 and Kidney Diseases
 Hypertension Antibodies  >523 publications with ICAM1 and Hypertension
 Asthma Antibodies  >502 publications with ICAM1 and Asthma
 Stroke Antibodies  >385 publications with ICAM1 and Stroke
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11766 publications with ICAM1 and Blood
 Vascular Antibodies  >8808 publications with ICAM1 and Vascular
 Lung Antibodies  >1773 publications with ICAM1 and Lung
 Heart Antibodies  >1284 publications with ICAM1 and Heart
 Skin Antibodies  >1196 publications with ICAM1 and Skin
 Liver Antibodies  >1177 publications with ICAM1 and Liver
 Kidney Antibodies  >1174 publications with ICAM1 and Kidney
 Brain Antibodies  >1174 publications with ICAM1 and Brain
 Muscle Antibodies  >1047 publications with ICAM1 and Muscle
 Embryonic Tissue Antibodies  >772 publications with ICAM1 and Embryonic Tissue
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