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NT elisa kit :: Porcine Nitrotyrosine ELISA Kit

Scan QR to view Datasheet Catalog #    MBS006965 NT elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Nitrotyrosine (NT), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Nitrotyrosine ELISA Kit

 Product Gene Name   

NT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q27995
 Species Reactivity    Porcine
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Assay Type    Sandwich (Quantitative)
 Detection Range    0.25 nmol/L - 8 nmol/L.
 Sensitivity    0.1 nmol/L.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NT purchase    MBS006965 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nitrotyrosine (NT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 nmol/L - 8 nmol/L in biological research samples containing NT, with an estimated sensitivity of 0.1 nmol/L. The ELISA analytical biochemical technique of the MBS006965 kit is based on NT antibody-NT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NT elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of NT (hereafter termed "analyte") in undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS006965. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NT. It may not necessarily be applicable to this product.
 NCBI GI #    116003827
 NCBI GeneID    282876
 NCBI Accession #    NP_001070267.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001076799.1 [Other Products]
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 UniProt Primary Accession #    Q27995 [Other Products]
 UniProt Secondary Accession #    Q06Q84; Q27985 [Other Products]
 UniProt Related Accession #    Q27995 [Other Products]
 Molecular Weight    131,208 Da
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 NCBI Official Full Name    nitric oxide synthase, inducible
 NCBI Official Synonym Full Names    nitric oxide synthase 2, inducible
 NCBI Official Symbol    NOS2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
 UniProt Protein Name    Nitric oxide synthase, inducible
 UniProt Synonym Protein Names   
Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2
 UniProt Gene Name    NOS2 [Similar Products]
 UniProt Synonym Gene Names    Inducible NOS; iNOS; NOSII [Similar Products]
 UniProt Entry Name    NOS2_BOVIN
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 UniProt Comments for NT    Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.
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 Research Articles on NT    1. Vitamin D receptor activation, and inducible nitric oxide synthase (NOS2), were strongly induced during Cooperia oncophora reinfection. Several canonical pathways associated with NOS2 were impacted.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NT elisa kitDiseases associated with NT elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2447 publications with NT and Inflammation
 Necrosis Antibodies  >2010 publications with NT and Necrosis
 Sepsis Antibodies  >565 publications with NT and Sepsis
 Intestinal Diseases Antibodies  >407 publications with NT and Intestinal Diseases
 Liver Diseases Antibodies  >374 publications with NT and Liver Diseases
 Reperfusion Injury Antibodies  >371 publications with NT and Reperfusion Injury
 Hypertension Antibodies  >297 publications with NT and Hypertension
 Endotoxemia Antibodies  >258 publications with NT and Endotoxemia
 Shock, Septic Antibodies  >247 publications with NT and Shock, Septic
 Kidney Diseases Antibodies  >222 publications with NT and Kidney Diseases
Organs/Tissues associated with NT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1179 publications with NT and Muscle
 Intestine Antibodies  >492 publications with NT and Intestine
 Ovary Antibodies  >48 publications with NT and Ovary
 Extraembryonic Tissue Antibodies  >6 publications with NT and Extraembryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with NT and Uncharacterized Tissue
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