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OPN elisa kit :: Porcine OPN ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2514012 OPN elisa kit
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

OPN, ELISA Kit

 Also Known As   

Porcine OPN (Osteopontin) ELISA Kit

 Product Gene Name   

OPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS2514012 COA    COA PDF
 OMIM    166490
 3D Structure    ModBase 3D Structure for P10451
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 Species Reactivity    Porcine
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 Specificity    This kit recognizes natural and recombinant Porcine OPN. No significant cross-reactivity or interference between Porcine OPN and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    37.5pg/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of OPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OPN purchase    MBS2514012 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OPN, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing OPN, with an estimated sensitivity of 37.5pg/mL. The ELISA analytical biochemical technique of the MBS2514012 kit is based on OPN antibody-OPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for OPN elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine OPN concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OPN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OPN. You can calculate the concentration of OPN in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2514012
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NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.
 NCBI GI #    91206462
 NCBI GeneID    6696
 NCBI Accession #    NP_001035147.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001040058.1 [Other Products]
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 UniProt Primary Accession #    P10451 [Other Products]
 UniProt Secondary Accession #    Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1 [Other Products]
 UniProt Related Accession #    P10451 [Other Products]
 Molecular Weight    33,843 Da
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 NCBI Official Full Name    osteopontin isoform OPN-a
 NCBI Official Synonym Full Names    secreted phosphoprotein 1
 NCBI Official Symbol    SPP1 [Similar Products]
 NCBI Official Synonym Symbols   
OPN; BNSP; BSPI; ETA-1
[Similar Products]
 NCBI Protein Information    osteopontin; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); urinary stone protein; uropontin
 UniProt Protein Name    Osteopontin
 UniProt Synonym Protein Names   
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
 UniProt Gene Name    SPP1 [Similar Products]
 UniProt Synonym Gene Names    BNSP; OPN; SPP-1 [Similar Products]
 UniProt Entry Name    OSTP_HUMAN
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 NCBI Summary for OPN    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for OPN    osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: osteoblast differentiation; neutrophil chemotaxis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; inflammatory response; cell adhesion; embryo implantation
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 Research Articles on OPN    1. SPP1 htSNP protects against low bone mineral density in middle-aged women
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Porcine OPN (Osteopontin) ELISA Kit
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Products associated with OPN elisa kitPathways associated with OPN elisa kit
 Reference Product  PubMed Publications
 CD44 elisa kit  >126 publications with OPN and CD44
 RUNX2 elisa kit  >122 publications with OPN and RUNX2
 BMP2 elisa kit  >73 publications with OPN and BMP2
 PTH elisa kit  >40 publications with OPN and PTH
 FN1 elisa kit  >11 publications with OPN and FN1
 ITGA5 elisa kit  >4 publications with OPN and ITGA5
 ITGB3 elisa kit  >3 publications with OPN and ITGB3
 ITGB1 elisa kit  >2 publications with OPN and ITGB1
 ITGAV elisa kit  >2 publications with OPN and ITGAV
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
Diseases associated with OPN elisa kitOrgans/Tissues associated with OPN elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >261 publications with OPN and Cardiovascular Diseases
 Inflammation Antibodies  >236 publications with OPN and Inflammation
 Kidney Diseases Antibodies  >228 publications with OPN and Kidney Diseases
 Fibrosis Antibodies  >150 publications with OPN and Fibrosis
 Neoplasm Metastasis Antibodies  >134 publications with OPN and Neoplasm Metastasis
 Necrosis Antibodies  >124 publications with OPN and Necrosis
 Calcinosis Antibodies  >111 publications with OPN and Calcinosis
 Adenocarcinoma Antibodies  >102 publications with OPN and Adenocarcinoma
 Heart Diseases Antibodies  >101 publications with OPN and Heart Diseases
 Liver Diseases Antibodies  >84 publications with OPN and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1066 publications with OPN and Bone
 Blood Antibodies  >526 publications with OPN and Blood
 Kidney Antibodies  >363 publications with OPN and Kidney
 Vascular Antibodies  >332 publications with OPN and Vascular
 Connective Tissue Antibodies  >327 publications with OPN and Connective Tissue
 Muscle Antibodies  >278 publications with OPN and Muscle
 Bone Marrow Antibodies  >187 publications with OPN and Bone Marrow
 Mouth Antibodies  >176 publications with OPN and Mouth
 Heart Antibodies  >128 publications with OPN and Heart
 Liver Antibodies  >123 publications with OPN and Liver
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