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OPN elisa kit :: Porcine Osteopontin (OPN) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023027 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $525 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,255 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteopontin (OPN), ELISA Kit

★Popular Item★
 Also Known As   

Osteopontin (OPN) ELISA Kit

 Product Synonym Names    SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
 Product Gene Name   

OPN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    166490
 3D Structure    ModBase 3D Structure for P10451
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 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN).
No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.781-50ng/mL
 Sensitivity    < 0.28ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OPN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OPN purchase    MBS2023027 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteopontin (OPN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing OPN, with an estimated sensitivity of < 0.28ng/mL. The ELISA analytical biochemical technique of the MBS2023027 kit is based on OPN antibody-OPN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OPN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OPN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OPN elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteopontin (OPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteopontin (OPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for OPN elisa kit    Tumor immunity; Bone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of OPN elisa kit    OPN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023027. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.
 NCBI GI #    189405
 NCBI GeneID    6696
 NCBI Accession #    AAA59974.1 [Other Products]
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 UniProt Primary Accession #    P10451 [Other Products]
 UniProt Secondary Accession #    Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1 [Other Products]
 UniProt Related Accession #    P10451 [Other Products]
 Molecular Weight    33,843 Da
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 NCBI Official Full Name    osteopontin
 NCBI Official Synonym Full Names    secreted phosphoprotein 1
 NCBI Official Symbol    SPP1 [Similar Products]
 NCBI Official Synonym Symbols   
OPN; BNSP; BSPI; ETA-1
[Similar Products]
 NCBI Protein Information    osteopontin
 UniProt Protein Name    Osteopontin
 UniProt Synonym Protein Names   
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
 UniProt Gene Name    SPP1 [Similar Products]
 UniProt Synonym Gene Names    BNSP; OPN; SPP-1 [Similar Products]
 UniProt Entry Name    OSTP_HUMAN
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 NCBI Summary for OPN    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for OPN    osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: osteoblast differentiation; neutrophil chemotaxis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; cell adhesion; inflammatory response; embryo implantation
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 Research Articles on OPN    1. This review focuses on the role of NHE1 and OPN in cardiac remodeling and emphasizes the signaling pathways implicating OPN in the NHE1-induced hypertrophic response.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OPN elisa kitPathways associated with OPN elisa kit
 Reference Product  PubMed Publications
 CD44 elisa kit  >126 publications with OPN and CD44
 RUNX2 elisa kit  >122 publications with OPN and RUNX2
 BMP2 elisa kit  >73 publications with OPN and BMP2
 PTH elisa kit  >40 publications with OPN and PTH
 FN1 elisa kit  >11 publications with OPN and FN1
 ITGA5 elisa kit  >4 publications with OPN and ITGA5
 ITGB3 elisa kit  >3 publications with OPN and ITGB3
 ITGB1 elisa kit  >2 publications with OPN and ITGB1
 ITGAV elisa kit  >2 publications with OPN and ITGAV
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
Diseases associated with OPN elisa kitOrgans/Tissues associated with OPN elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >261 publications with OPN and Cardiovascular Diseases
 Inflammation Antibodies  >236 publications with OPN and Inflammation
 Kidney Diseases Antibodies  >228 publications with OPN and Kidney Diseases
 Fibrosis Antibodies  >150 publications with OPN and Fibrosis
 Neoplasm Metastasis Antibodies  >134 publications with OPN and Neoplasm Metastasis
 Necrosis Antibodies  >124 publications with OPN and Necrosis
 Calcinosis Antibodies  >111 publications with OPN and Calcinosis
 Adenocarcinoma Antibodies  >102 publications with OPN and Adenocarcinoma
 Heart Diseases Antibodies  >101 publications with OPN and Heart Diseases
 Liver Diseases Antibodies  >84 publications with OPN and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1066 publications with OPN and Bone
 Blood Antibodies  >526 publications with OPN and Blood
 Kidney Antibodies  >363 publications with OPN and Kidney
 Vascular Antibodies  >332 publications with OPN and Vascular
 Connective Tissue Antibodies  >327 publications with OPN and Connective Tissue
 Muscle Antibodies  >278 publications with OPN and Muscle
 Bone Marrow Antibodies  >187 publications with OPN and Bone Marrow
 Mouth Antibodies  >176 publications with OPN and Mouth
 Heart Antibodies  >128 publications with OPN and Heart
 Liver Antibodies  >123 publications with OPN and Liver
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