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| Product Name
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S100 Calcium Binding Protein A12 (S100A12), ELISA Kit ★Popular Item★
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| Also Known As
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Porcine S100 Calcium Binding Protein A12 ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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603112
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| Species Reactivity
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Porcine
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A12 and all the analogues, therefore, cross reaction may still exist in some cases.
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| Samples
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Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive
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| Sensitivity
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1.0 pg/mL.
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of S100A12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for S100A12 purchase
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MBS740961 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A12 (S100A12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S100A12, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740961 kit is based on S100A12 antibody-S100A12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Intended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||S100A12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A12 antibody and an S100A12-HRP conjugate. The assay sample and buffer are incubated together with S100A12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A12 concentration since S100A12 from samples and S100A12-HRP conjugate compete for the anti-S100A12 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A12 from the sample, fewer sites are left to bind S100A12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for S100A12 elisa kit
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Signal Transduction
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| Typical Testing Data/Standard Curve of S100A12 elisa kit
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| Sample Manual Insert of MBS740961. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for S100A12. It may not necessarily be applicable to this product. |
| NCBI GI #
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119573717
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| NCBI GeneID
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6283
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| NCBI Accession #
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EAW53332.1
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[Other Products]
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| UniProt Secondary Accession #
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P83219; Q5SY66; Q7M4R1
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[Other Products]
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| UniProt Related Accession #
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P80511
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[Other Products]
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| Molecular Weight
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10,575 Da
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| NCBI Official Full Name
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S100 calcium binding protein A12 (calgranulin C)
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| NCBI Official Synonym Full Names
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S100 calcium binding protein A12
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| NCBI Official Symbol
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S100A12
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[Similar Products]
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| NCBI Official Synonym Symbols
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p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
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[Similar Products]
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| NCBI Protein Information
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protein S100-A12; EN-RAGE; calgranulin C; calgranulin-C; neutrophil S100 protein; calcium-binding protein in amniotic fluid 1; migration inhibitory factor-related protein 6; S100 calcium-binding protein A12 (calgranulin C); extracellular newly identified RAGE-binding protein
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| UniProt Protein Name
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Protein S100-A12
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| UniProt Synonym Protein Names
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CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Extracellular newly identified RAGE-binding protein; EN-RAGE; Migration inhibitory factor-related protein 6; MRP-6; p6; Neutrophil S100 protein; S100 calcium-binding protein A12Cleaved into the following chain:Calcitermin
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| Protein Family
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Protein
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| UniProt Gene Name
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S100A12
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[Similar Products]
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| UniProt Synonym Gene Names
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CAAF1; CAGC; EN-RAGE; MRP-6; p6
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[Similar Products]
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| UniProt Entry Name
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S10AC_HUMAN
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| NCBI Summary for S100A12
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The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq, Jul 2008]
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| UniProt Comments for S100A12
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S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family.
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoskeleton; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding
Biological Process: neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; mast cell activation; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of MAP kinase activity; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; cytokine secretion; positive regulation of inflammatory response
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| Research Articles on S100A12
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1. Excessive expression of the S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with cardiovascular disease.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with S100A12 elisa kit | Pathways associated with S100A12 elisa kit |
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| Diseases associated with S100A12 elisa kit | Organs/Tissues associated with S100A12 elisa kit |
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ELISA Kit 
