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AP-1 elisa kit :: Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS269963
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
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 Product Name   

Transcription Factor / Activator Protein 1 (AP-1), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

 Product Gene Name   

AP-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    165161
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 Species Reactivity    Porcine
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 Specificity    No cross-reaction with other factors.
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 Samples    Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-1 concentration
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156ng/ml
 Sensitivity    0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AP-1 purchase    MBS269963 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156ng/ml in biological research samples containing AP-1, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS269963 kit is based on AP-1 antibody-AP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-1 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AP-1 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine AP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of AP-1 elisa kit    AP-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS269963. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AP-1. It may not necessarily be applicable to this product.
 NCBI GI #    4504809
 NCBI GeneID    3726
 NCBI Accession #    NP_002220.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002229.2 [Other Products]
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 UniProt Secondary Accession #    Q96GH3 [Other Products]
 UniProt Related Accession #    P17275 [Other Products]
 Molecular Weight    35,879 Da
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 NCBI Official Full Name    transcription factor jun-B
 NCBI Official Synonym Full Names    jun B proto-oncogene
 NCBI Official Symbol    JUNB [Similar Products]
 NCBI Official Synonym Symbols   
AP-1
[Similar Products]
 NCBI Protein Information    transcription factor jun-B; activator protein 1
 UniProt Protein Name    Transcription factor jun-B
 UniProt Gene Name    JUNB [Similar Products]
 UniProt Entry Name    JUNB_HUMAN
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 UniProt Comments for AP-1    JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: response to drug; response to peptide hormone stimulus; response to light stimulus; transcription initiation from RNA polymerase II promoter; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; trophectodermal cell differentiation; cellular response to hormone stimulus; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; positive regulation of cell differentiation; vasculogenesis; response to corticosterone stimulus
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 Research Articles on AP-1    1. These results demonstrate a unique induction of JUNB in response to kinase inhibitor therapies that may be among the earliest events in the progression to treatment resistance.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AP-1 elisa kitPathways associated with AP-1 elisa kit
 Reference Product  PubMed Publications
 FOS elisa kit  >704 publications with AP-1 and FOS
 FOSB elisa kit  >204 publications with AP-1 and FOSB
 FOSL2 elisa kit  >35 publications with AP-1 and FOSL2
 SMAD4 elisa kit  >15 publications with AP-1 and SMAD4
 IL6 elisa kit  >14 publications with AP-1 and IL6
 MAPK8 elisa kit  >6 publications with AP-1 and MAPK8
 FOSL1 elisa kit  >4 publications with AP-1 and FOSL1
 EP300 elisa kit  >3 publications with AP-1 and EP300
 BCL6 elisa kit  >2 publications with AP-1 and BCL6
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
Diseases associated with AP-1 elisa kitOrgans/Tissues associated with AP-1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >81 publications with AP-1 and Inflammation
 Necrosis Antibodies  >68 publications with AP-1 and Necrosis
 Nervous System Diseases Antibodies  >51 publications with AP-1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >47 publications with AP-1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >44 publications with AP-1 and Cardiovascular Diseases
 Liver Diseases Antibodies  >37 publications with AP-1 and Liver Diseases
 Brain Diseases Antibodies  >35 publications with AP-1 and Brain Diseases
 Neoplasms, Experimental Antibodies  >34 publications with AP-1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >31 publications with AP-1 and Liver Neoplasms
 Lung Diseases Antibodies  >25 publications with AP-1 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >209 publications with AP-1 and Blood
 Brain Antibodies  >182 publications with AP-1 and Brain
 Bone Antibodies  >84 publications with AP-1 and Bone
 Liver Antibodies  >83 publications with AP-1 and Liver
 Skin Antibodies  >76 publications with AP-1 and Skin
 Nerve Antibodies  >59 publications with AP-1 and Nerve
 Lung Antibodies  >56 publications with AP-1 and Lung
 Vascular Antibodies  >56 publications with AP-1 and Vascular
 Bone Marrow Antibodies  >40 publications with AP-1 and Bone Marrow
 Kidney Antibodies  >33 publications with AP-1 and Kidney
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