View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List TF elisa kit :: Porcine Transferrin ELISA Kit Catalog # MBS737110 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Transferrin (TF), ELISA Kit Popular Item Full Product Name Porcine Transferrin ELISA Kit Product Gene Name TF elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual OMIM gene 209300 Species Reactivity Porcine Specificity This assay has high sensitivity and excellent specificity for detection of TFE. No significant cross-reactivity or interference between TFE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TFE and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 1.0 ug/mL Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of TF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for TF purchase MBS737110 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transferrin (TF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TF. The ELISA analytical biochemical technique of the MBS737110 kit is based on TF antibody-TF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for TF elisa kit Intended Uses: This TFE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TFE. This ELISA kit for research use only!Principle of the Assay: TFE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TFE antibody and an TFE-HRP conjugate. The assay sample and buffer are incubated together with TFE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFE concentration since TFE from samples and TFE-HRP conjugate compete for the anti-TFE antibody binding site. Since the number of sites is limited, as more sites are occupied by TFE from the sample, fewer sites are left to bind TFE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFE concentration in each sample is interpolated from this standard curve. Product Categories/Family for TF elisa kit Cardiovascular Typical Testing Data/Standard Curve (for reference only) of TF elisa kit TOP Sample Manual Insert of MBS737110. NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product. NCBI GI # 248648 NCBI GeneID 7018 NCBI Accession # AAB22049.1 [Other Products] UniProt Secondary Accession # O43890; Q1HBA5; Q9NQB8; Q9UHV0[Other Products] UniProt Related Accession # P02787[Other Products] Molecular Weight 77,064 Da TOP NCBI Official Full Name transferrin NCBI Official Synonym Full Names transferrin NCBI Official Symbol TF [Similar Products] NCBI Official Synonym Symbols TFQTL1; PRO1557; PRO2086 [Similar Products] NCBI Protein Information serotransferrin; siderophilin; beta-1 metal-binding globulin UniProt Protein Name Serotransferrin UniProt Synonym Protein Names Beta-1 metal-binding globulin; Siderophilin Protein Family Transferrin UniProt Gene Name TF [Similar Products] UniProt Synonym Gene Names PRO1400; Transferrin [Similar Products] UniProt Entry Name TRFE_HUMAN TOP NCBI Summary for TF This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009] TOP UniProt Comments for TF TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 3q22.1Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; perinuclear region of cytoplasm; endocytic vesicle; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicleMolecular Function: protein binding; ferric iron binding; ubiquitin protein ligase bindingBiological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transportDisease: Atransferrinemia Research Articles on TF 1. Elevated BMI reduces the diagnostic utility of carbohydrate-deficient transferrin at higher alcohol intake in subjects with liver disease. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with TF elisa kit Reference Product PubMed Publications ALB elisa kit >82 publications with TF and ALB HFE elisa kit >72 publications with TF and HFE TFR2 elisa kit >42 publications with TF and TFR2 TFRC elisa kit >21 publications with TF and TFRC TTR elisa kit >7 publications with TF and TTR SLC40A1 elisa kit >5 publications with TF and SLC40A1 IGFBP3 elisa kit >5 publications with TF and IGFBP3 APOA1 elisa kit >4 publications with TF and APOA1 Pathways associated with TF elisa kit Products by Pathway Pathway Diagram EPHB Forward Signaling Pathway antibodies EPHB Forward Signaling Pathway Diagram HIF-1 Signaling Pathway antibodies HIF-1 Signaling Pathway Diagram HIF-1-alpha Transcription Factor Network Pathway antibodies HIF-1-alpha Transcription Factor Network Pathway Diagram Hemostasis Pathway antibodies Hemostasis Pathway Diagram Iron Metabolism In Placenta Pathway antibodies Iron Metabolism In Placenta Pathway Diagram Iron Uptake And Transport Pathway antibodies Iron Uptake And Transport Pathway Diagram Mineral Absorption Pathway antibodies Mineral Absorption Pathway Diagram Mineral Absorption Pathway antibodies Mineral Absorption Pathway Diagram Platelet Activation, Signaling And Aggregation Pathway antibodies Platelet Activation, Signaling And Aggregation Pathway Diagram Platelet Degranulation Pathway antibodies Platelet Degranulation Pathway Diagram Diseases associated with TF elisa kit Disease Name Pubmed Publications Cardiovascular Diseases Antibodies >1445 publications with TF and Cardiovascular Diseases Inflammation Antibodies >777 publications with TF and Inflammation Nervous System Diseases Antibodies >663 publications with TF and Nervous System Diseases Carcinoma Antibodies >517 publications with TF and Carcinoma Necrosis Antibodies >493 publications with TF and Necrosis Immune System Diseases Antibodies >424 publications with TF and Immune System Diseases Atherosclerosis Antibodies >285 publications with TF and Atherosclerosis Liver Diseases Antibodies >239 publications with TF and Liver Diseases Lung Neoplasms Antibodies >171 publications with TF and Lung Neoplasms Anemia Antibodies >142 publications with TF and Anemia Organs/Tissues associated with TF elisa kit Organ/Tissue Name Pubmed Publications Blood Antibodies >4574 publications with TF and Blood Brain Antibodies >934 publications with TF and Brain Heart Antibodies >672 publications with TF and Heart Liver Antibodies >612 publications with TF and Liver Muscle Antibodies >540 publications with TF and Muscle Lung Antibodies >468 publications with TF and Lung Kidney Antibodies >436 publications with TF and Kidney Embryonic Tissue Antibodies >379 publications with TF and Embryonic Tissue Nerve Antibodies >206 publications with TF and Nerve Skin Antibodies >205 publications with TF and Skin TOP © 2006-24 MyBioSource.com. 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