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TNNI3 elisa kit :: Porcine Troponin I Type 3, Cardiac (TNNI3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS451467
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $570 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,580 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,020 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Troponin I Type 3, Cardiac (TNNI3), ELISA Kit

★Popular Item★
 Also Known As   

Porcine Troponin I Type 3, Cardiac (TNNI3) ELISA Kit

 Product Synonym Names    cTnI; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I
 Product Gene Name   

TNNI3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for A5X497
 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TNNI3. No significant cross-reactivity or interference between TNNI3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    12.6pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNNI3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNNI3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNNI3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNNI3 purchase    MBS451467 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Troponin I Type 3, Cardiac (TNNI3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing TNNI3, with an estimated sensitivity of 12.6pg/mL. The ELISA analytical biochemical technique of the MBS451467 kit is based on TNNI3 antibody-TNNI3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNNI3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNNI3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNNI3 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNNI3 in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNNI3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNNI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNNI3 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNNI3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TNNI3 elisa kit    TNNI3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS451467. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
 NCBI GI #    148747272
 NCBI GeneID    100049696
 NCBI Accession #    NP_001092069.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001098599.1 [Other Products]
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 UniProt Primary Accession #    A5X497 [Other Products]
 Molecular Weight    23,982 Da
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 NCBI Official Full Name    troponin I, cardiac muscle
 NCBI Official Symbol    TNNI3 [Similar Products]
 NCBI Protein Information    troponin I, cardiac muscle
 UniProt Protein Name    Cardiac troponin I
 UniProt Synonym Protein Names   
Cardiac troponin I
 Protein Family    Troponin
 UniProt Gene Name    TNNI3 [Similar Products]
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 Research Articles on TNNI3    1. We show that the phosphorylation of cTnI and alphaTm vary in the different chambers of the heart, whereas the phosphorylation of MLC2 and cTnT does not.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNNI3 elisa kitPathways associated with TNNI3 elisa kit
 Reference Product  PubMed Publications
 TNNT2 elisa kit  >71 publications with TNNI3 and TNNT2
 TNNC1 elisa kit  >18 publications with TNNI3 and TNNC1
 TNNT1 elisa kit  >2 publications with TNNI3 and TNNT1
 MLC2V elisa kit  >1 publications with TNNI3 and MLC2V
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Conduction Pathway antibodies  Cardiac Conduction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Ion Homeostasis Pathway antibodies  Ion Homeostasis Pathway Diagram
Diseases associated with TNNI3 elisa kitOrgans/Tissues associated with TNNI3 elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >98 publications with TNNI3 and Heart Diseases
 Cardiomyopathies Antibodies  >85 publications with TNNI3 and Cardiomyopathies
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >33 publications with TNNI3 and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >26 publications with TNNI3 and Cardiomyopathy, Dilated
 Cardiomegaly Antibodies  >26 publications with TNNI3 and Cardiomegaly
 Cardiomyopathy, Restrictive Antibodies  >12 publications with TNNI3 and Cardiomyopathy, Restrictive
 CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7 Antibodies  >10 publications with TNNI3 and CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7
 Nervous System Diseases Antibodies  >7 publications with TNNI3 and Nervous System Diseases
 Fibrosis Antibodies  >7 publications with TNNI3 and Fibrosis
 Ventricular Dysfunction, Left Antibodies  >6 publications with TNNI3 and Ventricular Dysfunction, Left
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >80 publications with TNNI3 and Heart
 Muscle Antibodies  >71 publications with TNNI3 and Muscle
 Embryonic Tissue Antibodies  >5 publications with TNNI3 and Embryonic Tissue
 Uterus Antibodies  >1 publications with TNNI3 and Uterus
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