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alpha1-AT elisa kit :: Porcine alpha1 Antitrypsin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735637
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha1 Antitrypsin (alpha1-AT), ELISA Kit

 Also Known As   

Porcine alpha1 Antitrypsin ELISA Kit

 Product Synonym Names    Porcine a1 Antitrypsin ELISA Kit
 Product Gene Name   

alpha1-AT elisa kit

[Similar Products]
 Product Synonym Gene Name    a1-AT [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Porcine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of alpha1AT. No significant cross-reactivity or interference between alpha1AT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha1AT and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of alpha1-AT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for alpha1-AT purchase    MBS735637 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha1 Antitrypsin (alpha1-AT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha1-AT, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS735637 kit is based on alpha1-AT antibody-alpha1-AT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha1-AT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha1-AT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for alpha1-AT elisa kit

   Intended Uses: This alpha1AT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine alpha1AT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: alpha1AT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-alpha1AT antibody and an alpha1AT-HRP conjugate. The assay sample and buffer are incubated together with alpha1AT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha1AT concentration since alpha1AT from samples and alpha1AT-HRP conjugate compete for the anti-alpha1AT antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha1AT from the sample, fewer sites are left to bind alpha1AT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1AT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for alpha1-AT elisa kit    Cardiovascular
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 Typical Standard Curve/Testing Data of alpha1-AT elisa kit    alpha1-AT elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS735637. Click to request current manual
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NCBI/Uniprot data below describe general gene information for alpha1-AT. It may not necessarily be applicable to this product.
 NCBI GI #    2804195
 NCBI Accession #    BAA24422.1 [Other Products]
 Molecular Weight    45,729 Da
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 NCBI Official Full Name    alpha1-antitrypsin
 UniProt Protein Name    Alpha-1-antiproteinase
 UniProt Synonym Protein Names   
Alpha-1-antitrypsin; Alpha-1-proteinase inhibitor
 UniProt Entry Name    A1AT_CALCN
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 UniProt Comments for alpha1-AT    Inhibitor of serine proteases.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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