Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CK-18 rightarrow Porcine CK-18  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • growth differentiation factor 11 (GDF11) ELISA Kit
 • Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit
 • Defensin Beta 2 (DEFb2) Antibody
 • extracellular signal-regulated kinase, ERK ELISA Kit
 • ZEB1 Blocking Peptide
 • Nerve Growth Factor Receptor (NGFR) Recombinant Protein
 • delta-like 3 (Drosophila) (DLL3) ELISA Kit
 • GHR-GHBP (Growth Hormone Receptor) CLIA Kit
 • Procainamide Antibody
 • NKG2-A-C-E Antibody
 • STAT1 Antibody
 • 14-3-3, 30, 33kD Antibody
 • ADAM metallopeptidase with thrombospondin type 1 motif, 13 (... ELISA Kit
 • Calcium Assay Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CK-18 elisa kit :: Porcine cytokeratin 18 (CK-18) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS264983
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cytokeratin 18 (CK-18), ELISA Kit

★Popular Item★
 Also Known As   

Porcine cytokeratin 18 (CK-18) ELISA Kit

 Product Gene Name   

CK-18 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 215600
 3D Structure    ModBase 3D Structure for P05783
Section Bar
 Species Reactivity    Porcine
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    Up to 0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CK-18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CK-18 purchase    MBS264983 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytokeratin 18 (CK-18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing CK-18, with an estimated sensitivity of Up to 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS264983 kit is based on CK-18 antibody-CK-18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK-18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CK-18 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine CK-18 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Standard Curve/Testing Data of CK-18 elisa kit    CK-18 elisa kit Typical Standard Curve/Testing Data image
Table BarTOPTable Bar
Sample Manual Insert of MBS264983. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CK-18. It may not necessarily be applicable to this product.
 NCBI GI #    34037
 NCBI GeneID    3875
 NCBI Accession #    CAA31375.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P05783 [Other Products]
 UniProt Secondary Accession #    Q53G38; Q5U0N8; Q9BW26 [Other Products]
 UniProt Related Accession #    P05783 [Other Products]
 Molecular Weight    48,058 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cytokeratin 18
 NCBI Official Synonym Full Names    keratin 18
 NCBI Official Symbol    KRT18 [Similar Products]
 NCBI Official Synonym Symbols   
K18; CYK18
[Similar Products]
 NCBI Protein Information    keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
 UniProt Protein Name    Keratin, type I cytoskeletal 18
 UniProt Synonym Protein Names   
Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
 UniProt Gene Name    KRT18 [Similar Products]
 UniProt Synonym Gene Names    CYK18; PIG46; CK-18; K18 [Similar Products]
 UniProt Entry Name    K1C18_HUMAN
Section Bar
 NCBI Summary for CK-18    KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CK-18    K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; intermediate filament; microtubule organizing center

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial
Table BarTOPTable Bar
 Research Articles on CK-18    1. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CK-18 elisa kitPathways associated with CK-18 elisa kit
 Reference Product  PubMed Publications
 KRT8 elisa kit  >42 publications with CK-18 and KRT8
 TCHP elisa kit  >1 publications with CK-18 and TCHP
 CASP6 elisa kit  >1 publications with CK-18 and CASP6
 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
Diseases associated with CK-18 elisa kitOrgans/Tissues associated with CK-18 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >44 publications with CK-18 and Neoplasms
 Liver Diseases Antibodies  >28 publications with CK-18 and Liver Diseases
 Carcinoma Antibodies  >25 publications with CK-18 and Carcinoma
 Breast Neoplasms Antibodies  >14 publications with CK-18 and Breast Neoplasms
 Adenocarcinoma Antibodies  >13 publications with CK-18 and Adenocarcinoma
 Fibrosis Antibodies  >12 publications with CK-18 and Fibrosis
 Neoplasm Metastasis Antibodies  >10 publications with CK-18 and Neoplasm Metastasis
 Fatty Liver Antibodies  >9 publications with CK-18 and Fatty Liver
 Liver Cirrhosis Antibodies  >8 publications with CK-18 and Liver Cirrhosis
 Liver Neoplasms Antibodies  >7 publications with CK-18 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >34 publications with CK-18 and Liver
 Blood Antibodies  >18 publications with CK-18 and Blood
 Eye Antibodies  >6 publications with CK-18 and Eye
 Muscle Antibodies  >6 publications with CK-18 and Muscle
 Lymph Antibodies  >6 publications with CK-18 and Lymph
 Lymph Node Antibodies  >6 publications with CK-18 and Lymph Node
 Kidney Antibodies  >6 publications with CK-18 and Kidney
 Prostate Antibodies  >5 publications with CK-18 and Prostate
 Stomach Antibodies  >5 publications with CK-18 and Stomach
 Vascular Antibodies  >4 publications with CK-18 and Vascular
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions