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F2 elisa kit :: Prothrombin total antigen assay ELISA Kit

Scan QR to view Datasheet Catalog #    MBS135499
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1 Kit  /  $365 +1 FREE 8GB USB
5 Kits  /  $1,450 +1 FREE 8GB USB
 
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 Product Name   

Prothrombin total antigen assay (F2), ELISA Kit

★Popular Item★
 Also Known As   

Human Prothrombin total antigen assay ELISA Kit

 Product Gene Name   

F2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 11; NC_000011.9 (46740743..46761056). Location: 11p11
 OMIM    176930
 3D Structure    ModBase 3D Structure for P00734
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 Host    Multiple
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 Specificity    This assay recognizes natural human total prothrombin (prothrombin, thrombin, and thrombin-antithrombin complex). Pooled normal plasma from mouse, pig, and canine were assayed for cross-reactivity. No significant cross-reactivity was observed.
 Form/Format    Complete Kit
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 Samples    Human plasma
 Sensitivity    The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.147-0.169) and calculating the corresponding concentration. The MDD was 0.166 ng/ml.
 Intra-assay Precision    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
 Inter-assay Precision    Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F2 purchase    MBS135499 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple Prothrombin total antigen assay (F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2, with an estimated sensitivity of The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.147-0.169) and calculating the corresponding concentration. The MDD was 0.166 ng/ml. The ELISA analytical biochemical technique of the MBS135499 kit is based on F2 antibody-F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for F2 elisa kit

   Intended Uses: This human prothrombin antigen assay is intended for the quantitative determination of total prothrombin antigen in human plasma. For research use only.

Principle of the Assay: Human prothrombin will bind to the capture antibody coated on the microtiter plate. Prothrombin, thrombin, and thrombin-antithrombin complex will react with the antibody on the plate. After appropriate washing steps, anti-human prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prothrombin. Color development is proportional to the concentration of total prothrombin in the samples.

Background: Prothrombin (aka Factor II) is a single-chain vitamin K-dependent 579 amino acid glycoprotein zymogen [1]. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease [2]. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210 [3].
 Product Categories/Family for F2 elisa kit    Thrombin/Prothrombin; Human Prothrombin ELISA Kits
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 Typical Testing Data/Standard Curve (for reference only) of F2 elisa kit    F2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS135499. Click to request current manual
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NCBI/Uniprot data below describe general gene information for F2. It may not necessarily be applicable to this product.
 NCBI GI #    4503635
 NCBI GeneID    2147
 NCBI Accession #    NP_000497.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000506.3 [Other Products]
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 UniProt Primary Accession #    P00734 [Other Products]
 UniProt Secondary Accession #    Q4QZ40; Q53H04; Q53H06; Q7Z7P3; Q9UCA1; B2R7F7; B4E1A7 [Other Products]
 UniProt Related Accession #    P00734 [Other Products]
 Molecular Weight    70,037 Da
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 NCBI Official Full Name    prothrombin preproprotein
 NCBI Official Synonym Full Names    coagulation factor II (thrombin)
 NCBI Official Symbol    F2 [Similar Products]
 NCBI Official Synonym Symbols   
PT; THPH1; RPRGL2
[Similar Products]
 NCBI Protein Information    prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
 UniProt Protein Name    Prothrombin
 UniProt Synonym Protein Names   
Coagulation factor II
 Protein Family    Prothrombin
 UniProt Gene Name    F2 [Similar Products]
 UniProt Entry Name    THRB_HUMAN
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 NCBI Summary for F2    Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F2    prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.5; Protease; Secreted; Apoptosis

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: protein binding; growth factor activity; serine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: positive regulation of blood coagulation; multicellular organismal development; positive regulation of collagen biosynthetic process; proteolysis; regulation of cell shape; negative regulation of fibrinolysis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; response to wounding; acute-phase response; negative regulation of proteolysis; platelet activation; cytosolic calcium ion homeostasis; post-translational protein modification; positive regulation of cell growth; peptidyl-glutamic acid carboxylation; positive regulation of phosphoinositide 3-kinase cascade; fibrinolysis; cellular protein metabolic process; regulation of gene expression; negative regulation of astrocyte differentiation; regulation of blood coagulation; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; leukocyte migration; blood coagulation, intrinsic pathway

Disease: Thrombophilia Due To Thrombin Defect; Stroke, Ischemic; Prothrombin Deficiency, Congenital; Pregnancy Loss, Recurrent, Susceptibility To, 2
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 Research Articles on F2    1. Spatial dynamics of thrombin was studied by developing a fluorogenic substrate-based method for time- and space-resolved imaging of thrombin enzymatic activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with F2 elisa kitPathways associated with F2 elisa kit
 Reference Product  PubMed Publications
 SERPINE1 elisa kit  >24 publications with F2 and SERPINE1
 PROC elisa kit  >21 publications with F2 and PROC
 F11 elisa kit  >17 publications with F2 and F11
 FGA elisa kit  >8 publications with F2 and FGA
 SPP1 elisa kit  >8 publications with F2 and SPP1
 IGFBP3 elisa kit  >5 publications with F2 and IGFBP3
 F2R elisa kit  >5 publications with F2 and F2R
 GP1BA elisa kit  >4 publications with F2 and GP1BA
 F13A1 elisa kit  >4 publications with F2 and F13A1
 IGFBP5 elisa kit  >3 publications with F2 and IGFBP5
 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Diseases associated with F2 elisa kitOrgans/Tissues associated with F2 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1029 publications with F2 and Nervous System Diseases
 Liver Diseases Antibodies  >694 publications with F2 and Liver Diseases
 Neoplasms Antibodies  >675 publications with F2 and Neoplasms
 Hypertension Antibodies  >656 publications with F2 and Hypertension
 Drug Toxicity Antibodies  >604 publications with F2 and Drug Toxicity
 Inflammation Antibodies  >515 publications with F2 and Inflammation
 Liver Cirrhosis Antibodies  >458 publications with F2 and Liver Cirrhosis
 Brain Diseases Antibodies  >449 publications with F2 and Brain Diseases
 Heart Diseases Antibodies  >297 publications with F2 and Heart Diseases
 Kidney Diseases Antibodies  >252 publications with F2 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1268 publications with F2 and Liver
 Uterus Antibodies  >953 publications with F2 and Uterus
 Kidney Antibodies  >709 publications with F2 and Kidney
 Heart Antibodies  >659 publications with F2 and Heart
 Embryonic Tissue Antibodies  >364 publications with F2 and Embryonic Tissue
 Connective Tissue Antibodies  >252 publications with F2 and Connective Tissue
 Spleen Antibodies  >229 publications with F2 and Spleen
 Bone Marrow Antibodies  >104 publications with F2 and Bone Marrow
 Mammary Gland Antibodies  >32 publications with F2 and Mammary Gland
 Uncharacterized Tissue Antibodies  >2 publications with F2 and Uncharacterized Tissue
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