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F2 elisa kit :: Prothrombin total antigen assay ELISA Kit

Scan QR to view Datasheet Catalog #    MBS135518
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1 Kit  /  $385 +1 FREE 8GB USB
5 Kits  /  $1,420 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Prothrombin total antigen assay (F2), ELISA Kit

 Also Known As   

Mouse Prothrombin total antigen assay ELISA Kit

 Product Gene Name   

F2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 2; NC_000068.7 (91625320..91636414, complement). Location: 2 E1; 2 50.63 cM
 3D Structure    ModBase 3D Structure for P19221
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 Host    Multiple
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 Specificity    This assay recognizes natural mouse prothrombin, thrombin, and thrombin/antithrombin complex. Significant cross reaction is observed with pooled normal plasma from rat. Pooled normal plasma from human, porcine, rabbit, and sheep were assayed for cross-reactivity. No significant cross-reactivity was observed.
 Form/Format    Complete Kit
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 Samples    Mouse plasma, serum, tissue extracts, and cell culture media
 Sensitivity    The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.114-0.124) and calculating the corresponding concentration. The MDD was 0.319 ng/ml.
 Intra-assay Precision    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
 Inter-assay Precision    Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C. Shelf Life: 6 months from manufacture, see label for expiration date
 Other Notes    Small volumes of F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F2 purchase    MBS135518 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple Prothrombin total antigen assay (F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2, with an estimated sensitivity of The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.114-0.124) and calculating the corresponding concentration. The MDD was 0.319 ng/ml. The ELISA analytical biochemical technique of the MBS135518 kit is based on F2 antibody-F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Mouse plasma, serum, tissue extracts, and cell culture media. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for F2 elisa kit

   Intended Uses: This mouse prothrombin/thrombin total antigen assay is intended for the quantitative determination of total prothrombin and thrombin in mouse plasma, serum, tissue extracts, and cell culture media. For quantitation of mouse prothrombin only, refer to the Mouse Prothrombin Antigen Assay (cat # MPTKT). For research use only.

Principle of the Assay: Mouse prothrombin, thrombin, and thrombin-antithrombin complex will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of prothrombin in the sample.

Background: Prothrombin (aka Factor II) is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen [1]. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease [2]. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210 [3].
 Product Categories/Family for F2 elisa kit    Thrombin/Prothrombin; Mouse Prothrombin ELISA Kits
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 Typical Testing Data/Standard Curve (for reference only) of F2 elisa kit    F2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS135518
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NCBI/Uniprot data below describe general gene information for F2. It may not necessarily be applicable to this product.
 NCBI GI #    6753798
 NCBI GeneID    14061
 NCBI Accession #    NP_034298.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010168.2 [Other Products]
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 UniProt Primary Accession #    P19221 [Other Products]
 UniProt Related Accession #    P19221 [Other Products]
 Molecular Weight    70,269 Da
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 NCBI Official Full Name    prothrombin
 NCBI Official Synonym Full Names    coagulation factor II
 NCBI Official Symbol    F2 [Similar Products]
 NCBI Official Synonym Symbols   
Cf2; FII; Cf-2; thrombin
[Similar Products]
 NCBI Protein Information    prothrombin
 UniProt Protein Name    Prothrombin
 UniProt Synonym Protein Names   
Coagulation factor II
 Protein Family    Prothrombin
 UniProt Gene Name    F2 [Similar Products]
 UniProt Synonym Gene Names    Cf2 [Similar Products]
 UniProt Entry Name    THRB_MOUSE
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 NCBI Summary for F2    This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]
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 UniProt Comments for F2    prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.5; Apoptosis

Cellular Component: extracellular matrix; extracellular space; cell; extracellular region; cytosol

Molecular Function: peptidase activity; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding; catalytic activity; receptor binding

Biological Process: negative regulation of proteolysis; platelet activation; positive regulation of blood coagulation; cytosolic calcium ion homeostasis; positive regulation of collagen biosynthetic process; proteolysis; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; fibrinolysis; cell surface receptor linked signal transduction; hemostasis; regulation of gene expression; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; acute-phase response; response to wounding; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation
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 Research Articles on F2    1. PKCalpha and Akt modulate platelet function by phosphorylating and inhibiting GSK3alpha/beta, thereby relieving the negative effect of GSK3alpha/beta on thrombin-mediated platelet activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with F2 elisa kitDiseases associated with F2 elisa kit
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1029 publications with F2 and Nervous System Diseases
 Liver Diseases Antibodies  >694 publications with F2 and Liver Diseases
 Neoplasms Antibodies  >675 publications with F2 and Neoplasms
 Hypertension Antibodies  >656 publications with F2 and Hypertension
 Drug Toxicity Antibodies  >604 publications with F2 and Drug Toxicity
 Inflammation Antibodies  >515 publications with F2 and Inflammation
 Liver Cirrhosis Antibodies  >458 publications with F2 and Liver Cirrhosis
 Brain Diseases Antibodies  >449 publications with F2 and Brain Diseases
 Heart Diseases Antibodies  >297 publications with F2 and Heart Diseases
 Kidney Diseases Antibodies  >252 publications with F2 and Kidney Diseases
Organs/Tissues associated with F2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1941 publications with F2 and Muscle
 Liver Antibodies  >1268 publications with F2 and Liver
 Brain Antibodies  >1086 publications with F2 and Brain
 Lung Antibodies  >666 publications with F2 and Lung
 Embryonic Tissue Antibodies  >364 publications with F2 and Embryonic Tissue
 Intestine Antibodies  >269 publications with F2 and Intestine
 Thymus Antibodies  >95 publications with F2 and Thymus
 Lymph Node Antibodies  >56 publications with F2 and Lymph Node
 Extraembryonic Tissue Antibodies  >33 publications with F2 and Extraembryonic Tissue
 Salivary Gland Antibodies  >31 publications with F2 and Salivary Gland
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