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5HT elisa kit :: Rabbit 5 Hydroxytryptamine ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733558
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

5 Hydroxytryptamine (5HT), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit 5 Hydroxytryptamine ELISA Kit

 Product Synonym Names    Rabbit 5 Hydroxytryptamine ELISA Kit; 5 Hydroxytryptamine; 5 Hydroxytryptamine ELISA Kit (Rabbit)
 Product Gene Name   

5HT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS733558 Testing Data    Testing Data PDF
 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of 5-HT. No significant cross-reactivity or interference between 5-HT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 5-HT and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of 5HT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for 5HT purchase    MBS733558 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5 Hydroxytryptamine (5HT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5HT, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS733558 kit is based on 5HT antibody-5HT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5HT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5HT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for 5HT elisa kit

   Intended Uses: This 5-HT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit 5-HT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||5-HT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-5-HT antibody and an 5-HT-HRP conjugate. The assay sample and buffer are incubated together with 5-HT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5-HT concentration since 5-HT from samples and 5-HT-HRP conjugate compete for the anti-5-HT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5-HT from the sample, fewer sites are left to bind 5-HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5-HT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for 5HT elisa kit    Rabbit ELISA Kit
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 Typical Testing Data/Standard Curve of 5HT elisa kit    5HT elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS733558. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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