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AAP elisa kit :: Rabbit AAP ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505808
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


 Also Known As   

Rabbit AAP (Alanine Aminopeptidase) ELISA Kit

 Product Gene Name   

AAP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    262850
 3D Structure    ModBase 3D Structure for P08697
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 Species Reactivity    Rabbit
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 Specificity    This kit recognizes natural and recombinant Rabbit AAP. No significant cross-reactivity or interference between Rabbit AAP and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.625-40ng/mL
 Sensitivity    Min: 0.375ng/mL; Max: 40ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AAP purchase    MBS2505808 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AAP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing AAP, with an estimated sensitivity of Min: 0.375ng/mL; Max: 40ng/mL. The ELISA analytical biochemical technique of the MBS2505808 kit is based on AAP antibody-AAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AAP elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit AAP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AAP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AAP. You can calculate the concentration of AAP in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AAP elisa kit    AAP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505808. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AAP. It may not necessarily be applicable to this product.
 NCBI GI #    260064048
 NCBI GeneID    5345
 NCBI Accession #    NP_001159392.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001165920.1 [Other Products]
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 UniProt Primary Accession #    P08697 [Other Products]
 UniProt Secondary Accession #    Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7 [Other Products]
 UniProt Related Accession #    P08697 [Other Products]
 Molecular Weight    47,907 Da
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 NCBI Official Full Name    alpha-2-antiplasmin isoform a
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
 NCBI Official Symbol    SERPINF2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    alpha-2-antiplasmin; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin F2
 UniProt Protein Name    Alpha-2-antiplasmin
 UniProt Synonym Protein Names   
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
 UniProt Gene Name    SERPINF2 [Similar Products]
 UniProt Synonym Gene Names    AAP; PLI; Alpha-2-AP; Alpha-2-PI [Similar Products]
 UniProt Entry Name    A2AP_HUMAN
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 NCBI Summary for AAP    This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for AAP    SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation

Disease: Alpha-2-plasmin Inhibitor Deficiency
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 Research Articles on AAP    1. Two differentially expressed proteins, alpha-1-antitrypsin (SERPINA1) and alpha-2 antiplasmin (SERPINF2) are associated with purpura fulminans.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AAP elisa kitPathways associated with AAP elisa kit
 Reference Product  PubMed Publications
 PLAT elisa kit  >1 publications with AAP and PLAT
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
Diseases associated with AAP elisa kitOrgans/Tissues associated with AAP elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3 publications with AAP and Cardiovascular Diseases
 Fibrosis Antibodies  >3 publications with AAP and Fibrosis
 Liver Diseases Antibodies  >3 publications with AAP and Liver Diseases
 Inflammation Antibodies  >3 publications with AAP and Inflammation
 Blood Coagulation Disorders Antibodies  >2 publications with AAP and Blood Coagulation Disorders
 Hemorrhage Antibodies  >1 publications with AAP and Hemorrhage
 Hepatitis Antibodies  >1 publications with AAP and Hepatitis
 Kidney Diseases Antibodies  >1 publications with AAP and Kidney Diseases
 Liver Neoplasms Antibodies  >1 publications with AAP and Liver Neoplasms
 Brain Diseases Antibodies  >1 publications with AAP and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with AAP and Blood
 Liver Antibodies  >4 publications with AAP and Liver
 Brain Antibodies  >1 publications with AAP and Brain
 Kidney Antibodies  >1 publications with AAP and Kidney
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