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AGPA elisa kit :: Rabbit Anti-Glycoprotein 210 Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720233
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Glycoprotein 210 Antibody (AGPA), ELISA Kit

 Also Known As   

Rabbit Anti-Glycoprotein 210 Antibody ELISA Kit

 Product Gene Name   

AGPA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607703
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GP210Ab. No significant cross-reactivity or interference between GP210Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GP210Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000ng/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This GP210Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit GP210Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AGPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AGPA purchase    MBS720233 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Glycoprotein 210 Antibody (AGPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000ng/mL in biological research samples containing AGPA, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS720233 kit is based on AGPA antibody-AGPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AGPA elisa kit

   Principle of the assay: GP210Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing GP210 antigen and an GP210Ab-HRP conjugate. The assay sample and buffer are incubated together with GP210Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GP210Ab concentration since GP210Ab from samples and GP210Ab-HRP conjugate compete for the GP210 antigen binding site. Since the number of sites is limited, as more sites are occupied by GP210Ab from the sample, fewer sites are left to bind GP210Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP210Ab concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AGPA elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of AGPA elisa kit    AGPA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS720233. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AGPA. It may not necessarily be applicable to this product.
 NCBI GI #    27477134
 NCBI GeneID    23225
 NCBI Accession #    NP_079199.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_024923.3 [Other Products]
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 UniProt Secondary Accession #    O94980; Q6NXG6; Q8NBJ1; Q9H6C8; Q9UFP3; A6NN56 [Other Products]
 UniProt Related Accession #    Q8TEM1 [Other Products]
 Molecular Weight    105,810 Da
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 NCBI Official Full Name    nuclear pore membrane glycoprotein 210
 NCBI Official Synonym Full Names    nucleoporin 210kDa
 NCBI Official Symbol    NUP210 [Similar Products]
 NCBI Official Synonym Symbols   
GP210; POM210
[Similar Products]
 NCBI Protein Information    nuclear pore membrane glycoprotein 210; nucleoporin Nup210; nuclear pore protein gp210; pore membrane protein of 210 kDa; nuclear envelope pore membrane protein POM 210
 UniProt Protein Name    Nuclear pore membrane glycoprotein 210
 UniProt Synonym Protein Names   
Nuclear envelope pore membrane protein POM 210; POM210; Nucleoporin Nup210; Pore membrane protein of 210 kDa
 Protein Family    Periplasmic alpha-galactoside-binding protein
 UniProt Gene Name    NUP210 [Similar Products]
 UniProt Synonym Gene Names    KIAA0906; PSEC0245; Nuclear pore protein gp210; POM210 [Similar Products]
 UniProt Entry Name    PO210_HUMAN
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 NCBI Summary for AGPA    The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]
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 UniProt Comments for AGPA    NUP210: Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity. Belongs to the NUP210 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; nuclear pore; nuclear envelope

Molecular Function: protein dimerization activity

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport
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 Research Articles on AGPA    1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AGPA elisa kitPathways associated with AGPA elisa kit
 Reference Product  PubMed Publications
 NUP214 elisa kit  >2 publications with AGPA and NUP214
 NUP153 elisa kit  >2 publications with AGPA and NUP153
 NUP107 elisa kit  >1 publications with AGPA and NUP107
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Export Of Viral Ribonucleoproteins From Nucleus Pathway antibodies  Export Of Viral Ribonucleoproteins From Nucleus Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucose Transport Pathway antibodies  Glucose Transport Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
Diseases associated with AGPA elisa kitOrgans/Tissues associated with AGPA elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >41 publications with AGPA and Liver Diseases
 Liver Cirrhosis Antibodies  >40 publications with AGPA and Liver Cirrhosis
 Hepatitis Antibodies  >10 publications with AGPA and Hepatitis
 Liver Failure Antibodies  >7 publications with AGPA and Liver Failure
 Inflammation Antibodies  >5 publications with AGPA and Inflammation
 Mitochondrial Diseases Antibodies  >2 publications with AGPA and Mitochondrial Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with AGPA and Carcinoma, Hepatocellular
 Necrosis Antibodies  >2 publications with AGPA and Necrosis
 Liver Neoplasms, Experimental Antibodies  >1 publications with AGPA and Liver Neoplasms, Experimental
 Fibrosis Antibodies  >1 publications with AGPA and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >42 publications with AGPA and Liver
 Blood Antibodies  >24 publications with AGPA and Blood
 Kidney Antibodies  >3 publications with AGPA and Kidney
 Embryonic Tissue Antibodies  >3 publications with AGPA and Embryonic Tissue
 Connective Tissue Antibodies  >1 publications with AGPA and Connective Tissue
 Cervix Antibodies  >1 publications with AGPA and Cervix
 Heart Antibodies  >1 publications with AGPA and Heart
 Stomach Antibodies  >1 publications with AGPA and Stomach
 Spleen Antibodies  >1 publications with AGPA and Spleen
 Nerve Antibodies  >1 publications with AGPA and Nerve
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