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RNP elisa kit :: Rabbit Anti-Ribonucleoprotein Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725194
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Ribonucleoprotein Antibody (RNP), ELISA Kit

 Also Known As   

Rabbit Anti-Ribonucleoprotein Antibody ELISA Kit

 Product Gene Name   

RNP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    160994
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Anti-RNP. No significant cross-reactivity or interference between Anti-RNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-RNP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    10-250 U/mL
 Sensitivity    0.1 U/mL
 Intended Uses    This Anti-RNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit Anti-RNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RNP purchase    MBS725194 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Ribonucleoprotein Antibody (RNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10-250 U/mL in biological research samples containing RNP, with an estimated sensitivity of 0.1 U/mL. The ELISA analytical biochemical technique of the MBS725194 kit is based on RNP antibody-RNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RNP elisa kit

   Principle of the assay: Anti-RNP ELISA kit applies the competitive enzyme immunoassay technique utilizing RNP antigen and an Anti-RNP-HRP conjugate. The assay sample and buffer are incubated together with Anti-RNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-RNP concentration since Anti-RNP from samples and Anti-RNP-HRP conjugate compete for the RNP antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-RNP from the sample, fewer sites are left to bind Anti-RNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-RNP concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for RNP elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of RNP elisa kit    RNP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS725194. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RNP. It may not necessarily be applicable to this product.
 NCBI GI #    16905456
 NCBI GeneID    4670
 NCBI Accession #    AAL31359.1 [Other Products]
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 UniProt Secondary Accession #    Q15584; Q8WZ44; Q96H56; Q9BWL9; Q9Y492 [Other Products]
 UniProt Related Accession #    P52272 [Other Products]
 Molecular Weight    – Da
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 NCBI Official Full Name    ribonucleoprotein
 NCBI Official Synonym Full Names    heterogeneous nuclear ribonucleoprotein M
 NCBI Official Symbol    HNRNPM [Similar Products]
 NCBI Official Synonym Symbols   
CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
[Similar Products]
 NCBI Protein Information    heterogeneous nuclear ribonucleoprotein M; CEA receptor; hnRNA-binding protein M4; N-acetylglucosamine receptor 1; heterogenous nuclear ribonucleoprotein M4
 UniProt Protein Name    Heterogeneous nuclear ribonucleoprotein M
 UniProt Gene Name    HNRNPM [Similar Products]
 UniProt Synonym Gene Names    HNRPM; NAGR1; hnRNP M [Similar Products]
 UniProt Entry Name    HNRPM_HUMAN
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 NCBI Summary for RNP    This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
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 UniProt Comments for RNP    hnRNP M: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; Nucleolus; RNA splicing; RNA-binding; Receptor, misc.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; spliceosome; extracellular matrix; nuclear matrix; membrane; integral to plasma membrane; nucleolus; paraspeckles

Molecular Function: protein domain specific binding; protein binding; RNA binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
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 Research Articles on RNP    1. findings demonstrate a novel molecular mechanism through which tumor metastasis is endowed by the hnRNPM-mediated splicing program.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RNP elisa kitPathways associated with RNP elisa kit
 Reference Product  PubMed Publications
 HNRNPU elisa kit  >2 publications with RNP and HNRNPU
 HNRNPK elisa kit  >2 publications with RNP and HNRNPK
 HNRNPL elisa kit  >2 publications with RNP and HNRNPL
 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
Diseases associated with RNP elisa kitOrgans/Tissues associated with RNP elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2 publications with RNP and Carcinoma
 Liver Neoplasms Antibodies  >2 publications with RNP and Liver Neoplasms
 Breast Neoplasms Antibodies  >1 publications with RNP and Breast Neoplasms
 Liver Diseases Antibodies  >1 publications with RNP and Liver Diseases
 Necrosis Antibodies  >1 publications with RNP and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >2 publications with RNP and Thyroid
 Kidney Antibodies  >2 publications with RNP and Kidney
 Liver Antibodies  >2 publications with RNP and Liver
 Blood Antibodies  >1 publications with RNP and Blood
 Nerve Antibodies  >1 publications with RNP and Nerve
 Pancreas Antibodies  >1 publications with RNP and Pancreas
 Lung Antibodies  >1 publications with RNP and Lung
 Placenta Antibodies  >1 publications with RNP and Placenta
 Muscle Antibodies  >1 publications with RNP and Muscle
 Brain Antibodies  >1 publications with RNP and Brain
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