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BNP elisa kit :: Rabbit Brain Natriuretic Peptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731058
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Brain Natriuretic Peptide (BNP), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Brain Natriuretic Peptide ELISA Kit

 Product Gene Name   

BNP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS731058 COA    COA PDF
 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BNP and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BNP purchase    MBS731058 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Brain Natriuretic Peptide (BNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BNP, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS731058 kit is based on BNP antibody-BNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BNP elisa kit

   Intended Uses: This BNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit BNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: BNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BNP antibody and an BNP-HRP conjugate. The assay sample and buffer are incubated together with BNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BNP concentration since BNP from samples and BNP-HRP conjugate compete for the anti-BNP antibody binding site. Since the number of sites is limited, as more sites are occupied by BNP from the sample, fewer sites are left to bind BNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BNP concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for BNP elisa kit    Neurobiology
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  Typical Testing Data/Standard Curve (for reference only) of BNP elisa kit    BNP elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731058. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BNP. It may not necessarily be applicable to this product.
 NCBI GI #    74096009
 NCBI GeneID    446068
 NCBI Accession #    NP_001027860.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001032688.1 [Other Products]
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 UniProt Related Accession #    Q805D7 [Other Products]
 Molecular Weight    14,746 Da
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 NCBI Official Full Name    brain natriuretic peptide
 NCBI Official Symbol    nppb [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    brain natriuretic peptide
 UniProt Protein Name    Brain natriuretic peptide
 UniProt Synonym Protein Names   
B-type natriuretic peptide
 Protein Family    Brain natriuretic peptide
 UniProt Gene Name    nppb [Similar Products]
 UniProt Synonym Gene Names    bnp [Similar Products]
 UniProt Entry Name    ANFB_TAKRU
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 UniProt Comments for BNP    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Has a cGMP-stimulating activity ().
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BNP elisa kitDiseases associated with BNP elisa kit
 Reference Product  PubMed Publications
 NPR3 elisa kit  >5 publications with BNP and NPR3
 IDE elisa kit  >1 publications with BNP and IDE
 CYP1A2 elisa kit  >1 publications with BNP and CYP1A2
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >138 publications with BNP and Fibrosis
 Cardiovascular Diseases Antibodies  >72 publications with BNP and Cardiovascular Diseases
 Heart Diseases Antibodies  >36 publications with BNP and Heart Diseases
 Hypertension Antibodies  >26 publications with BNP and Hypertension
 Hypertrophy Antibodies  >18 publications with BNP and Hypertrophy
 Edema Antibodies  >15 publications with BNP and Edema
 Heart Failure Antibodies  >15 publications with BNP and Heart Failure
 Inflammation Antibodies  >14 publications with BNP and Inflammation
 Necrosis Antibodies  >13 publications with BNP and Necrosis
 Postoperative Complications Antibodies  >12 publications with BNP and Postoperative Complications
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