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C3a elisa kit :: Rabbit Complement Fragment 3a ELISA Kit

Scan QR to view Datasheet Catalog #    MBS748816
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Fragment 3a (C3a), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Complement Fragment 3a ELISA Kit

 Product Gene Name   

C3a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613779
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C3alpha. No significant cross-reactivity or interference between C3alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C3alpha and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive (Quantitative)
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C3a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C3a purchase    MBS748816 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Fragment 3a (C3a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C3a, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS748816 kit is based on C3a antibody-C3a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C3a elisa kit

   Intended Uses: This C3alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RabbitC3alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: C3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3alpha antibody and an C3alpha-HRP conjugate. The assay sample and buffer are incubated together with C3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3alpha concentration since C3alpha from samples and C3alpha-HRP conjugate compete for the anti-C3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by C3alpha from the sample, fewer sites are left to bind C3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3alpha concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for C3a elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of C3a elisa kit    C3a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS748816. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C3a. It may not necessarily be applicable to this product.
 NCBI GI #    115298678
 NCBI GeneID    718
 NCBI Accession #    NP_000055.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000064.2 [Other Products]
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 UniProt Secondary Accession #    A7E236 [Other Products]
 UniProt Related Accession #    P01024 [Other Products]
 Molecular Weight    187,148 Da
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 NCBI Official Full Name    complement C3
 NCBI Official Synonym Full Names    complement component 3
 NCBI Official Symbol    C3 [Similar Products]
 NCBI Official Synonym Symbols   
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
[Similar Products]
 NCBI Protein Information    complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; epididymis secretory sperm binding protein Li 62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
 UniProt Protein Name    Complement C3
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1Cleaved into the following 11 chains:Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg
 UniProt Gene Name    C3 [Similar Products]
 UniProt Synonym Gene Names    CPAMD1; ASP [Similar Products]
 UniProt Entry Name    CO3_HUMAN
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 NCBI Summary for C3a    Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Feb 2009]
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 UniProt Comments for C3a    C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway

Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
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 Research Articles on C3a    1. have identified Complement C3 as a candidate plasma biomarker for measuring disease state in neuroblastoma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C3a elisa kitPathways associated with C3a elisa kit
 Reference Product  PubMed Publications
 CR1 elisa kit  >424 publications with C3a and CR1
 CR2 elisa kit  >293 publications with C3a and CR2
 CFH elisa kit  >206 publications with C3a and CFH
 CD46 elisa kit  >193 publications with C3a and CD46
 CFI elisa kit  >73 publications with C3a and CFI
 ITGAM elisa kit  >71 publications with C3a and ITGAM
 ITGAX elisa kit  >18 publications with C3a and ITGAX
 CFD elisa kit  >4 publications with C3a and CFD
 C3AR1 elisa kit  >3 publications with C3a and C3AR1
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
Diseases associated with C3a elisa kitOrgans/Tissues associated with C3a elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >3637 publications with C3a and Kidney Diseases
 Cardiovascular Diseases Antibodies  >2390 publications with C3a and Cardiovascular Diseases
 Inflammation Antibodies  >1915 publications with C3a and Inflammation
 Proteinuria Antibodies  >1269 publications with C3a and Proteinuria
 Lung Diseases Antibodies  >1011 publications with C3a and Lung Diseases
 Pain Antibodies  >989 publications with C3a and Pain
 Brain Diseases Antibodies  >941 publications with C3a and Brain Diseases
 Necrosis Antibodies  >927 publications with C3a and Necrosis
 Liver Diseases Antibodies  >922 publications with C3a and Liver Diseases
 Hemorrhage Antibodies  >771 publications with C3a and Hemorrhage
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11797 publications with C3a and Blood
 Kidney Antibodies  >3488 publications with C3a and Kidney
 Bone Antibodies  >2905 publications with C3a and Bone
 Skin Antibodies  >2260 publications with C3a and Skin
 Brain Antibodies  >2092 publications with C3a and Brain
 Liver Antibodies  >1786 publications with C3a and Liver
 Muscle Antibodies  >1679 publications with C3a and Muscle
 Spinal Cord Antibodies  >1564 publications with C3a and Spinal Cord
 Nerve Antibodies  >1337 publications with C3a and Nerve
 Lung Antibodies  >1090 publications with C3a and Lung
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