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DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

GABA elisa kit :: Rabbit Gamma-aminobutyric acid ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722440
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gamma-aminobutyric acid (GABA), ELISA Kit

 Also Known As   

Rabbit Gamma-aminobutyric acid ELISA Kit

 Product Synonym Names    Rabbit Gamma-aminobutyric acid ELISA Kit; Gamma-aminobutyric acid; Gamma-aminobutyric acid (Rabbit)
 Product Gene Name   

GABA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Gamma-aminobutyric acid. No significant cross-reactivity or interference between Gamma-aminobutyric acid and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Gamma-aminobutyric acid and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 nmol/ml.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GABA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GABA purchase    MBS722440 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gamma-aminobutyric acid (GABA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GABA, with an estimated sensitivity of 0.1 nmol/ml. The ELISA analytical biochemical technique of the MBS722440 kit is based on GABA antibody-GABA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GABA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GABA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GABA elisa kit

   Intended Uses: This Gamma-aminobutyric acid ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit Gamma-aminobutyric acid. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||Gamma-aminobutyric acid ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-Gamma-aminobutyric acid antibody and an Gamma-aminobutyric acid-HRP conjugate. The assay sample and buffer are incubated together with Gamma-aminobutyric acid-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Gamma-aminobutyric acid concentration since Gamma-aminobutyric acid from samples and Gamma-aminobutyric acid-HRP conjugate compete for the anti-Gamma-aminobutyric acid antibody binding site. Since the number of sites is limited, as more sites are occupied by Gamma-aminobutyric acid from the sample, fewer sites are left to bind Gamma-aminobutyric acid-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Gamma-aminobutyric acid concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for GABA elisa kit    Rabbit ELISA Kit
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 Typical Testing Data/Standard Curve of GABA elisa kit    GABA elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS722440. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GABA. It may not necessarily be applicable to this product.
 NCBI GI #    170592096
 NCBI GeneID    6104228
 NCBI GenBank Nucleotide #    XP_001900805.1 [Other Products]
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 NCBI Official Full Name    Gaba
 NCBI Official Symbol    Bm1_46715 [Similar Products]
 NCBI Protein Information    Gaba
 Protein Family    Gamma-aminobutyric acid receptor
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GABA elisa kitOrgans/Tissues associated with GABA elisa kit
 Products by Pathway  Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with GABA and Bone
 Connective Tissue Antibodies  >4 publications with GABA and Connective Tissue
 Heart Antibodies  >3 publications with GABA and Heart
 Muscle Antibodies  >3 publications with GABA and Muscle
 Brain Antibodies  >3 publications with GABA and Brain
 Vascular Antibodies  >2 publications with GABA and Vascular
 Embryonic Tissue Antibodies  >2 publications with GABA and Embryonic Tissue
 Ear Antibodies  >1 publications with GABA and Ear
 Kidney Antibodies  >1 publications with GABA and Kidney
 Umbilical Cord Antibodies  >1 publications with GABA and Umbilical Cord
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