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IHH elisa kit :: Rabbit Hedgehog Homolog, Indian ELISA Kit

Scan QR to view Datasheet Catalog #    MBS746766
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Hedgehog Homolog, Indian (IHH), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Hedgehog Homolog, Indian ELISA Kit

 Product Gene Name   

IHH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IHH. No significant cross-reactivity or interference between IHH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IHH and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IHH purchase    MBS746766 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hedgehog Homolog, Indian (IHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IHH, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS746766 kit is based on IHH antibody-IHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IHH elisa kit

   Intended Uses: This IHH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IHH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||IHH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IHH antibody and an IHH-HRP conjugate. The assay sample and buffer are incubated together with IHH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IHH concentration since IHH from samples and IHH-HRP conjugate compete for the anti-IHH antibody binding site. Since the number of sites is limited, as more sites are occupied by IHH from the sample, fewer sites are left to bind IHH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHH concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of IHH elisa kit    IHH elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS746766. Click to request current manual
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 Protein Family    Indian hedgehog protein
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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