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IL-10 elisa kit :: Rabbit Interleukin-10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764535
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin-10 (IL-10), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Interleukin-10 ELISA Kit

 Product Gene Name   

IL-10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764535 COA    COA PDF
 MBS764535 QC Report    QC Report PDF
 OMIM    124092
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-10. No significant cross-reactivity or interference between IL-10 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    15.625-1000pg/ml
 Sensitivity    <9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-10 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL- 10 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-10 purchase    MBS764535 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-10 (IL-10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing IL-10, with an estimated sensitivity of <9.375pg/ml. The ELISA analytical biochemical technique of the MBS764535 kit is based on IL-10 antibody-IL-10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-10 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of IL-10 elisa kit    IL-10 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS764535. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.
 NCBI GI #    10835141
 NCBI GeneID    3586
 NCBI Accession #    NP_000563.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000572.2 [Other Products]
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 UniProt Related Accession #    P22301 [Other Products]
 Molecular Weight    20,517 Da
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 NCBI Official Full Name    interleukin-10
 NCBI Official Synonym Full Names    interleukin 10
 NCBI Official Symbol    IL10 [Similar Products]
 NCBI Official Synonym Symbols   
CSIF; TGIF; GVHDS; IL-10; IL10A
[Similar Products]
 NCBI Protein Information    interleukin-10
 UniProt Protein Name    Interleukin-10
 UniProt Synonym Protein Names   
Cytokine synthesis inhibitory factor; CSIF
 Protein Family    Interleukin
 UniProt Gene Name    IL10 [Similar Products]
 UniProt Synonym Gene Names    IL-10; CSIF [Similar Products]
 UniProt Entry Name    IL10_HUMAN
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 NCBI Summary for IL-10    The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
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 UniProt Comments for IL-10    IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Secreted; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; interleukin-10 receptor binding; protein binding

Biological Process: aging; B cell differentiation; B cell proliferation; cell-cell signaling; cytoplasmic sequestering of NF-kappaB; defense response to bacterium; defense response to protozoan; hemopoiesis; inflammatory response; leukocyte chemotaxis; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; negative regulation of cytokine secretion during immune response; negative regulation of interferon-alpha biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-1 production; negative regulation of interleukin-12 production; negative regulation of interleukin-18 production; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of MHC class II biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of T cell proliferation; negative regulation of tumor necrosis factor biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptosis; positive regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; receptor biosynthetic process; regulation of gene expression; regulation of isotype switching; regulation of sensory perception of pain; response to activity; response to drug; response to glucocorticoid stimulus; response to inactivity; response to insulin stimulus; response to molecule of bacterial origin; T-helper 2 type immune response

Disease: Graft-versus-host Disease, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To; Rheumatoid Arthritis
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 Research Articles on IL-10    1. There was no association between IL-10 gene polymorphisms and juvenile-onset systemic lupus erythematosus in an Iranian cohort.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-10 elisa kitPathways associated with IL-10 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >2449 publications with IL-10 and JUN
 FOXP3 elisa kit  >1360 publications with IL-10 and FOXP3
 IL6 elisa kit  >910 publications with IL-10 and IL6
 IL4 elisa kit  >537 publications with IL-10 and IL4
 IL2 elisa kit  >307 publications with IL-10 and IL2
 IL8 elisa kit  >287 publications with IL-10 and IL8
 GATA3 elisa kit  >84 publications with IL-10 and GATA3
 STAT6 elisa kit  >72 publications with IL-10 and STAT6
 IL10RB elisa kit  >59 publications with IL-10 and IL10RB
 IL10RA elisa kit  >35 publications with IL-10 and IL10RA
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
Diseases associated with IL-10 elisa kitOrgans/Tissues associated with IL-10 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8758 publications with IL-10 and Necrosis
 Inflammation Antibodies  >7372 publications with IL-10 and Inflammation
 Sepsis Antibodies  >1285 publications with IL-10 and Sepsis
 Colitis Antibodies  >1054 publications with IL-10 and Colitis
 Genetic Predisposition to Disease Antibodies  >1019 publications with IL-10 and Genetic Predisposition to Disease
 Hypersensitivity, Immediate Antibodies  >900 publications with IL-10 and Hypersensitivity, Immediate
 Inflammatory Bowel Diseases Antibodies  >870 publications with IL-10 and Inflammatory Bowel Diseases
 Arthritis, Rheumatoid Antibodies  >642 publications with IL-10 and Arthritis, Rheumatoid
 Kidney Diseases Antibodies  >536 publications with IL-10 and Kidney Diseases
 HIV Infections Antibodies  >455 publications with IL-10 and HIV Infections
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17530 publications with IL-10 and Blood
 Lung Antibodies  >2019 publications with IL-10 and Lung
 Spleen Antibodies  >2008 publications with IL-10 and Spleen
 Connective Tissue Antibodies  >287 publications with IL-10 and Connective Tissue
 Uncharacterized Tissue Antibodies  >2 publications with IL-10 and Uncharacterized Tissue
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