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IL5 elisa kit :: Rabbit Interleukin-5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS284607 IL5 elisa kit
Unit / Price
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $790 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,500 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,655 +8 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin-5 (IL5), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Interleukin-5 (IL5) ELISA Kit

 Product Synonym Names    EDF; IL-5; TRF; B cell differentiation factor I|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5
 Product Gene Name   

IL5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    147850
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rabbit IL5. No significant cross-reactivity or interference between Rabbit IL5 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL5 purchase    MBS284607 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-5 (IL5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL5. The ELISA analytical biochemical technique of the MBS284607 kit is based on IL5 antibody-IL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL5 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL5 in samples. An antibody specific for IL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for IL5. It may not necessarily be applicable to this product.
 NCBI GI #    4504671
 NCBI GeneID    3567
 NCBI Accession #    NP_000870.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000879.2 [Other Products]
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 UniProt Secondary Accession #    Q13840 [Other Products]
 UniProt Related Accession #    P05113 [Other Products]
 Molecular Weight    15,238 Da
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 NCBI Official Full Name    interleukin-5
 NCBI Official Synonym Full Names    interleukin 5
 NCBI Official Symbol    IL5 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    interleukin-5
 UniProt Protein Name    Interleukin-5
 UniProt Synonym Protein Names   
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
 Protein Family    Interleukin
 UniProt Gene Name    IL5 [Similar Products]
 UniProt Synonym Gene Names    IL-5; TRF [Similar Products]
 UniProt Entry Name    IL5_HUMAN
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 NCBI Summary for IL5    This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
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 UniProt Comments for IL5    IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding

Biological Process: activation of MAPKK activity; axon guidance; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway
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 Research Articles on IL5    1. IL-5 may be part of protective mechanisms operating in early atherosclerosis, at least in women
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL5 elisa kitPathways associated with IL5 elisa kit
 Reference Product  PubMed Publications
 IL4 elisa kit  >233 publications with IL5 and IL4
 IL2 elisa kit  >105 publications with IL5 and IL2
 IL3 elisa kit  >52 publications with IL5 and IL3
 JUN elisa kit  >39 publications with IL5 and JUN
 IL5RA elisa kit  >27 publications with IL5 and IL5RA
 IL9 elisa kit  >26 publications with IL5 and IL9
 GATA3 elisa kit  >23 publications with IL5 and GATA3
 STAT6 elisa kit  >10 publications with IL5 and STAT6
 CSF2RB elisa kit  >5 publications with IL5 and CSF2RB
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Diseases associated with IL5 elisa kitOrgans/Tissues associated with IL5 elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >148 publications with IL5 and Hypersensitivity
 Inflammation Antibodies  >132 publications with IL5 and Inflammation
 Asthma Antibodies  >127 publications with IL5 and Asthma
 Lung Diseases Antibodies  >102 publications with IL5 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >101 publications with IL5 and Respiratory Hypersensitivity
 Eosinophilia Antibodies  >80 publications with IL5 and Eosinophilia
 Necrosis Antibodies  >67 publications with IL5 and Necrosis
 Fibrosis Antibodies  >16 publications with IL5 and Fibrosis
 Hyperplasia Antibodies  >14 publications with IL5 and Hyperplasia
 Bronchial Hyperreactivity Antibodies  >13 publications with IL5 and Bronchial Hyperreactivity
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >9 publications with IL5 and Thymus
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