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IL-6 elisa kit :: Rabbit Interleukin 6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS029352 IL-6 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 6 (IL-6), ELISA Kit

 Also Known As   

Rabbit Interleukin 6 ELISA Kit

 Product Gene Name   

IL-6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 4; NC_005103.3 (3095536..3100112). Location: 4q11
 3D Structure    ModBase 3D Structure for P20607
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 Species Reactivity    Rabbit
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 Specificity    No significant cross-reactivity or interference between Rabbit IL-6 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    6.25 pg/ml - 200 pg/ml.
 Sensitivity    1.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-6 concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit IL-6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-6 and IL-6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-6 purchase    MBS029352 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 6 (IL-6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing IL-6, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS029352 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-6 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-6 concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit IL-6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-6 and IL-6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS029352. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-6. It may not necessarily be applicable to this product.
 NCBI GI #    7549769
 NCBI GeneID    24498
 NCBI Accession #    NP_036721.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012589.2 [Other Products]
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 UniProt Primary Accession #    P20607 [Other Products]
 UniProt Related Accession #    P20607 [Other Products]
 Molecular Weight    24,357 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    Il6 [Similar Products]
 NCBI Official Synonym Symbols   
ILg6; Ifnb2
[Similar Products]
 NCBI Protein Information    interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
 UniProt Protein Name    Interleukin-6
 UniProt Gene Name    Il6 [Similar Products]
 UniProt Synonym Gene Names    Il-6; IL-6 [Similar Products]
 UniProt Entry Name    IL6_RAT
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 NCBI Summary for IL-6    a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
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 UniProt Comments for IL-6    Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-6 superfamily.
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 Research Articles on IL-6    1. Aerobic exercise training prevented the development of insulin resistance through IL-6 to a certain degree.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-6 elisa kitPathways associated with IL-6 elisa kit
 Reference Product  PubMed Publications
 Tnf elisa kit  >1112 publications with IL-6 and Tnf
 Crp elisa kit  >354 publications with IL-6 and Crp
 Stat3 elisa kit  >258 publications with IL-6 and Stat3
 Rela elisa kit  >43 publications with IL-6 and Rela
 Il6st elisa kit  >38 publications with IL-6 and Il6st
 Fos elisa kit  >36 publications with IL-6 and Fos
 Il12b elisa kit  >28 publications with IL-6 and Il12b
 Nfkb1 elisa kit  >23 publications with IL-6 and Nfkb1
 Csf2 elisa kit  >18 publications with IL-6 and Csf2
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with IL-6 elisa kitOrgans/Tissues associated with IL-6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1701 publications with IL-6 and Inflammation
 Necrosis Antibodies  >1307 publications with IL-6 and Necrosis
 Autoimmune Diseases Antibodies  >345 publications with IL-6 and Autoimmune Diseases
 Arthritis, Rheumatoid Antibodies  >217 publications with IL-6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >189 publications with IL-6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >182 publications with IL-6 and Myocardial Ischemia
 Ischemia Antibodies  >95 publications with IL-6 and Ischemia
 Myocardial Infarction Antibodies  >84 publications with IL-6 and Myocardial Infarction
 Hypersensitivity Antibodies  >82 publications with IL-6 and Hypersensitivity
 Diabetes Mellitus, Type 2 Antibodies  >79 publications with IL-6 and Diabetes Mellitus, Type 2
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL-6 and Uncharacterized Tissue
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