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MMP13 elisa kit :: Rabbit Matrix Metalloproteinase 13 (MMP13) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS450001
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $395 +1 FREE 8GB USB
96-Strip-Wells  /  $525 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,380 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,620 +5 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Matrix Metalloproteinase 13 (MMP13), ELISA Kit

 Also Known As   

Rabbit Matrix Metalloproteinase 13 (MMP13) ELISA Kit

 Product Synonym Names    CLG3; Collagenase 3
 Product Gene Name   

MMP13 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for O62806
 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MMP13. No significant cross-reactivity or interference between MMP13 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    3.125-200ng/mL
 Sensitivity    1.29ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP13 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP13 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP13 purchase    MBS450001 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix Metalloproteinase 13 (MMP13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/mL in biological research samples containing MMP13, with an estimated sensitivity of 1.29ng/mL. The ELISA analytical biochemical technique of the MBS450001 kit is based on MMP13 antibody-MMP13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP13 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP13 in rabbit serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MMP13 elisa kit    MMP13 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS450001. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP13. It may not necessarily be applicable to this product.
 NCBI GI #    126723537
 NCBI GeneID    100008685
 NCBI Accession #    NP_001075506.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001082037.1 [Other Products]
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 UniProt Primary Accession #    O62806 [Other Products]
 UniProt Related Accession #    O62806 [Other Products]
 Molecular Weight    53,693 Da
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 NCBI Official Full Name    collagenase 3
 NCBI Official Symbol    MMP13 [Similar Products]
 NCBI Protein Information    collagenase 3
 UniProt Protein Name    Collagenase 3
 UniProt Synonym Protein Names   
Matrix metalloproteinase-13; MMP-13
 Protein Family    Collagenase
 UniProt Gene Name    MMP13 [Similar Products]
 UniProt Synonym Gene Names    MMP-13 [Similar Products]
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 UniProt Comments for MMP13    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().
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 Research Articles on MMP13    1. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP13 elisa kitDiseases associated with MMP13 elisa kit
 Reference Product  PubMed Publications
 TNF elisa kit  >120 publications with MMP13 and TNF
 TIMP1 elisa kit  >75 publications with MMP13 and TIMP1
 TIMP2 elisa kit  >32 publications with MMP13 and TIMP2
 ETS1 elisa kit  >6 publications with MMP13 and ETS1
 TNC elisa kit  >4 publications with MMP13 and TNC
 POSTN elisa kit  >4 publications with MMP13 and POSTN
 S100B elisa kit  >2 publications with MMP13 and S100B
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >261 publications with MMP13 and Inflammation
 Disease Models, Animal Antibodies  >211 publications with MMP13 and Disease Models, Animal
 Necrosis Antibodies  >185 publications with MMP13 and Necrosis
 Fibrosis Antibodies  >120 publications with MMP13 and Fibrosis
 Neoplasm Invasiveness Antibodies  >86 publications with MMP13 and Neoplasm Invasiveness
 Liver Diseases Antibodies  >79 publications with MMP13 and Liver Diseases
 Hypertrophy Antibodies  >78 publications with MMP13 and Hypertrophy
 Breast Neoplasms Antibodies  >58 publications with MMP13 and Breast Neoplasms
 Pain Antibodies  >39 publications with MMP13 and Pain
 Atherosclerosis Antibodies  >31 publications with MMP13 and Atherosclerosis
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