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OLFM4 elisa kit :: Rabbit Olfactomedin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729500
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Olfactomedin 4 (OLFM4), ELISA Kit

 Also Known As   

Rabbit Olfactomedin 4 ELISA Kit

 Product Gene Name   

OLFM4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614061
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of OLFM4. No significant cross-reactivity or interference between OLFM4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OLFM4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This OLFM4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit OLFM4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of OLFM4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for OLFM4 purchase    MBS729500 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Olfactomedin 4 (OLFM4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing OLFM4, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS729500 kit is based on OLFM4 antibody-OLFM4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OLFM4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OLFM4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for OLFM4 elisa kit

   Principle of the assay: OLFM4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OLFM4 antibody and an OLFM4-HRP conjugate. The assay sample and buffer are incubated together with OLFM4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OLFM4 concentration since OLFM4 from samples and OLFM4-HRP conjugate compete for the anti-OLFM4 antibody binding site. Since the number of sites is limited, as more sites are occupied by OLFM4 from the sample, fewer sites are left to bind OLFM4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLFM4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for OLFM4 elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of OLFM4 elisa kit    OLFM4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729500. Click to request current manual
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NCBI/Uniprot data below describe general gene information for OLFM4. It may not necessarily be applicable to this product.
 NCBI GI #    32313593
 NCBI GeneID    10562
 NCBI Accession #    NP_006409.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_006418.4 [Other Products]
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 UniProt Secondary Accession #    O95362; Q5VWG0; Q86T22 [Other Products]
 UniProt Related Accession #    Q6UX06 [Other Products]
 Molecular Weight    57,280 Da
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 NCBI Official Full Name    olfactomedin-4
 NCBI Official Synonym Full Names    olfactomedin 4
 NCBI Official Symbol    OLFM4 [Similar Products]
 NCBI Official Synonym Symbols   
GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
[Similar Products]
 NCBI Protein Information    olfactomedin-4; antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein
 UniProt Protein Name    Olfactomedin-4
 UniProt Synonym Protein Names   
Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD
 Protein Family    Olfactomedin
 UniProt Gene Name    OLFM4 [Similar Products]
 UniProt Synonym Gene Names    GW112; UNQ362/PRO698; OLM4; hGC-1 [Similar Products]
 UniProt Entry Name    OLFM4_HUMAN
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 NCBI Summary for OLFM4    This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]
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 UniProt Comments for OLFM4    OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: extracellular space; specific granule; azurophil granule; mitochondrion; perinuclear region of cytoplasm; plasma membrane; secretory granule

Molecular Function: protein homodimerization activity; cadherin binding; catalytic activity

Biological Process: regulation of apoptosis; metabolic process; negative regulation of immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of phagocytosis; cell adhesion; protein homooligomerization
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 Research Articles on OLFM4    1. OLFM4 is a novel candidate tumor-suppressor gene for chromosome 13q and may shed new light on strategies that could be used for the diagnosis, prognosis, and treatment of prostate cancer patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with OLFM4 elisa kitDiseases associated with OLFM4 elisa kit
 Reference Product  PubMed Publications
 LGR5 elisa kit  >17 publications with OLFM4 and LGR5
 DEFA4 elisa kit  >2 publications with OLFM4 and DEFA4
 NOD2 elisa kit  >1 publications with OLFM4 and NOD2
 NOD1 elisa kit  >1 publications with OLFM4 and NOD1
 Disease Name  Pubmed Publications
 Pancreatic Neoplasms Antibodies  >5 publications with OLFM4 and Pancreatic Neoplasms
 Kidney Diseases Antibodies  >4 publications with OLFM4 and Kidney Diseases
 Prostatic Diseases Antibodies  >3 publications with OLFM4 and Prostatic Diseases
 Lung Neoplasms Antibodies  >3 publications with OLFM4 and Lung Neoplasms
 Lung Diseases Antibodies  >3 publications with OLFM4 and Lung Diseases
 Fibrosis Antibodies  >2 publications with OLFM4 and Fibrosis
 Hyperplasia Antibodies  >2 publications with OLFM4 and Hyperplasia
 Vascular Diseases Antibodies  >1 publications with OLFM4 and Vascular Diseases
 Necrosis Antibodies  >1 publications with OLFM4 and Necrosis
 Endometrial Neoplasms Antibodies  >1 publications with OLFM4 and Endometrial Neoplasms
Organs/Tissues associated with OLFM4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >19 publications with OLFM4 and Intestine
 Stomach Antibodies  >16 publications with OLFM4 and Stomach
 Prostate Antibodies  >6 publications with OLFM4 and Prostate
 Kidney Antibodies  >5 publications with OLFM4 and Kidney
 Nerve Antibodies  >2 publications with OLFM4 and Nerve
 Cervix Antibodies  >1 publications with OLFM4 and Cervix
 Testis Antibodies  >1 publications with OLFM4 and Testis
 Pancreas Antibodies  >1 publications with OLFM4 and Pancreas
 Uterus Antibodies  >1 publications with OLFM4 and Uterus
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