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PPARgamma elisa kit :: Rabbit Peroxisome Proliferators Activated Receptor gamma ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720587
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxisome Proliferators Activated Receptor gamma (PPARgamma), ELISA Kit

 Also Known As   

Rabbit Peroxisome Proliferators Activated Receptor gamma ELISA Kit

 Product Synonym Names    Rabbit Peroxisome Proliferators Activated Receptor g ELISA Kit
 Product Gene Name   

PPARgamma elisa kit

[Similar Products]
 Product Synonym Gene Name    PPARg [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rabbit
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PPARgamma elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPARgamma purchase    MBS720587 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxisome Proliferators Activated Receptor gamma (PPARgamma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing PPARgamma, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS720587 kit is based on PPARgamma antibody-PPARgamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPARgamma antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPARgamma. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PPARgamma elisa kit

   For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This PPARgamma ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PPARgamma in the sample, this PPARgamma ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PPARgamma concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PPARgamma utilizes a multiclonal anti-PPARgamma antibody and an PPARgamma-HRP conjugate. The assay sample and buffer are incubated together with PPARgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARgamma concentration since PPARgamma from samples and PPARgamma-HRP conjugate compete for the anti-PPARgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARgamma from the sample, fewer sites are left to bind PPARgamma-HRP conjugate. Standards of known PPARgamma concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of PPARgamma. The PPARgamma concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PPARgamma elisa kit    PPARgamma elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS720587. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PPARgamma. It may not necessarily be applicable to this product.
 NCBI GI #    147904405
 NCBI GeneID    100049069
 NCBI Accession #    NP_001091096.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001097627.1 [Other Products]
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 NCBI Official Full Name    peroxisome proliferators-activated receptor gamma
 NCBI Official Symbol    pparg [Similar Products]
 NCBI Protein Information    peroxisome proliferators-activated receptor gamma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PPARgamma elisa kit
 Products by Pathway  Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
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