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VEGF elisa kit :: Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700061
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vascular Endothelial cell Growth Factor, VEGF, ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

 Product Synonym Names    Rabbit Vascular Endothelial cell Growth Factor; VEGF ELISA Kit; Vascular Endothelial cell Growth Factor; VEGF
 Product Gene Name   

VEGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700061 Testing Data    Testing Data PDF
 OMIM    gene 603933
 3D Structure    ModBase 3D Structure for P15692
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 Species Reactivity    Rabbit
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rabbit VEGF. No significant cross-reactivity or interference between rabbit VEGF and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    1.56 pg/ml -100 pg/ml
 Sensitivity    Typically less than 0.39 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VEGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VEGF purchase    MBS700061 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vascular Endothelial cell Growth Factor, VEGF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 pg/ml -100 pg/ml in biological research samples containing VEGF, with an estimated sensitivity of Typically less than 0.39 pg/ml. The ELISA analytical biochemical technique of the MBS700061 kit is based on VEGF antibody-VEGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for VEGF elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of VEGF elisa kit    VEGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700061. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VEGF. It may not necessarily be applicable to this product.
 NCBI GI #    181971
 NCBI GeneID    7422
 NCBI Accession #    AAA35789.1 [Other Products]
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 UniProt Primary Accession #    P15692 [Other Products]
 UniProt Secondary Accession #    O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7 [Other Products]
 UniProt Related Accession #    P15692 [Other Products]
 Molecular Weight    34,406 Da
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 NCBI Official Full Name    vascular endothelial growth factor
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    VEGFA [Similar Products]
 NCBI Official Synonym Symbols   
VPF; VEGF; MVCD1
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 UniProt Gene Name    VEGFA [Similar Products]
 UniProt Synonym Gene Names    VEGF; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_HUMAN
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 NCBI Summary for VEGF    This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VEGF    Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.3 PublicationsManual assertion based on experiment in:Ref.27
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 Research Articles on VEGF    1. Plasma VEGF was significantly reduced in low birth weight (LBW) neonates while that of sVEGFR-1 and PF4 were significantly higher. VEGF/sVEGF/PF4 pathway seems to be involved in the endothelial progenitor cell dysfunction in LBW neonates.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGF elisa kitPathways associated with VEGF elisa kit
 Reference Product  PubMed Publications
 HIF1A elisa kit  >320 publications with VEGF and HIF1A
 KDR elisa kit  >202 publications with VEGF and KDR
 FLT1 elisa kit  >104 publications with VEGF and FLT1
 NRP1 elisa kit  >36 publications with VEGF and NRP1
 VEGFB elisa kit  >34 publications with VEGF and VEGFB
 CTGF elisa kit  >18 publications with VEGF and CTGF
 AKT1 elisa kit  >17 publications with VEGF and AKT1
 PGF elisa kit  >16 publications with VEGF and PGF
 NRP2 elisa kit  >12 publications with VEGF and NRP2
 SEMA3F elisa kit  >5 publications with VEGF and SEMA3F
 Products by Pathway  Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
Diseases associated with VEGF elisa kitOrgans/Tissues associated with VEGF elisa kit
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1761 publications with VEGF and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1247 publications with VEGF and Retinal Diseases
 Macular Degeneration Antibodies  >842 publications with VEGF and Macular Degeneration
 Neoplasm Metastasis Antibodies  >456 publications with VEGF and Neoplasm Metastasis
 Choroidal Neovascularization Antibodies  >430 publications with VEGF and Choroidal Neovascularization
 Inflammation Antibodies  >419 publications with VEGF and Inflammation
 Diabetic Retinopathy Antibodies  >394 publications with VEGF and Diabetic Retinopathy
 Lung Diseases Antibodies  >367 publications with VEGF and Lung Diseases
 Macular Edema Antibodies  >292 publications with VEGF and Macular Edema
 Heart Diseases Antibodies  >257 publications with VEGF and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >4946 publications with VEGF and Vascular
 Blood Antibodies  >1973 publications with VEGF and Blood
 Eye Antibodies  >808 publications with VEGF and Eye
 Bone Antibodies  >332 publications with VEGF and Bone
 Lung Antibodies  >326 publications with VEGF and Lung
 Muscle Antibodies  >324 publications with VEGF and Muscle
 Embryonic Tissue Antibodies  >304 publications with VEGF and Embryonic Tissue
 Heart Antibodies  >245 publications with VEGF and Heart
 Kidney Antibodies  >214 publications with VEGF and Kidney
 Skin Antibodies  >195 publications with VEGF and Skin
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