Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow TNF rightarrow Rabbit TNF  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Cytochrome P450c21 - 21-hydroxylase (CYP21A) ELISA Kit
 • Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit
 • EHHADH Antibody
 • TAB3 Antibody
 • Flt3-Ligand (Flt3l) Active Protein
 • CD79b Antibody
 • Complement C1q subcomponent subunit A (C1QA) Recombinant Protein
 • EFNB1-2 (EFNB2) Antibody
 • Immunoglobulin G (IgG) Secondary Antibody
 • CX3CR1 Antibody
 • CA10 Antibody
 • DEPDC5 cDNA Clone
 • Major Histocompatibility Complex Class I E (MHCE) Recombinant Protein
 • PICP Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

TNF elisa kit :: Rabbit tumor necrosis factor (TNF superfamily, member 2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700705
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tumor necrosis factor (TNF superfamily, member 2), ELISA Kit

★Popular Item★
 Also Known As   

Rabbit Tumor necrosis factor alpha, TNFalpha ELISA Kit

 Product Synonym Names    Rabbit Tumor necrosis factor alpha; TNFalpha ELISA Kit; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily; member 2)
 Product Gene Name   

TNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700705 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P04924
 Species Reactivity    Rabbit
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of rabbit TNFalpha. No significant cross-reactivity or interference between rabbit TNFalpha and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Sandwich
 Detection Range    78 pg/ml -5000 pg/ml.
 Sensitivity    19.5 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNF purchase    MBS700705 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tumor necrosis factor (TNF superfamily, member 2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 pg/ml -5000 pg/ml in biological research samples containing TNF, with an estimated sensitivity of 19.5 pg/ml. The ELISA analytical biochemical technique of the MBS700705 kit is based on TNF antibody-TNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for TNF elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFalpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFalpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFalpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFalpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of TNF elisa kit    TNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS700705. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    126722916
 NCBI GeneID    100009088
 NCBI Accession #    NP_001075732.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001082263.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P04924 [Other Products]
 UniProt Related Accession #    P04924 [Other Products]
 Molecular Weight    25,817 Da
Table BarTOPTable Bar
 NCBI Official Full Name    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
TNFA; TNFSF2
[Similar Products]
 NCBI Protein Information    tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_RABIT
Section Bar
 UniProt Comments for TNF    Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Table BarTOPTable Bar
 Research Articles on TNF    1. The TNFalpha-evoked Cl- current.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with TNF elisa kitPathways associated with TNF elisa kit
 Reference Product  PubMed Publications
 VCAM-1 elisa kit  >2484 publications with TNF and VCAM-1
 PTGS2 elisa kit  >2113 publications with TNF and PTGS2
 CD40 elisa kit  >1930 publications with TNF and CD40
 TGF-beta-1 elisa kit  >1769 publications with TNF and TGF-beta-1
 IL6 elisa kit  >1340 publications with TNF and IL6
 IL8 elisa kit  >395 publications with TNF and IL8
 eNOS elisa kit  >348 publications with TNF and eNOS
 REL elisa kit  >305 publications with TNF and REL
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with TNF elisa kitOrgans/Tissues associated with TNF elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >87971 publications with TNF and Necrosis
 Inflammation Antibodies  >31113 publications with TNF and Inflammation
 Vascular Diseases Antibodies  >7249 publications with TNF and Vascular Diseases
 Lung Diseases Antibodies  >6502 publications with TNF and Lung Diseases
 Liver Diseases Antibodies  >4918 publications with TNF and Liver Diseases
 Insulin Resistance Antibodies  >2393 publications with TNF and Insulin Resistance
 Fibrosis Antibodies  >2375 publications with TNF and Fibrosis
 Atherosclerosis Antibodies  >2305 publications with TNF and Atherosclerosis
 Colitis Antibodies  >2212 publications with TNF and Colitis
 Pain Antibodies  >2140 publications with TNF and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >42510 publications with TNF and Blood
 Lung Antibodies  >7680 publications with TNF and Lung
 Brain Antibodies  >5125 publications with TNF and Brain
 Heart Antibodies  >3861 publications with TNF and Heart
 Intestine Antibodies  >2807 publications with TNF and Intestine
 Connective Tissue Antibodies  >1769 publications with TNF and Connective Tissue
 Lymph Node Antibodies  >1653 publications with TNF and Lymph Node
 Placenta Antibodies  >698 publications with TNF and Placenta
 Prostate Antibodies  >648 publications with TNF and Prostate
 Ovary Antibodies  >468 publications with TNF and Ovary
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions