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DHVD3 elisa kit :: Rat 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2601701
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
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 Product Name   

1,25-Dihydroxyvitamin D3 (DHVD3), ELISA Kit

★Popular Item★
 Also Known As   

Rat 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

 Product Gene Name   

DHVD3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2601701 COA    COA PDF
 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    0.05ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DHVD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DHVD3 purchase    MBS2601701 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing DHVD3, with an estimated sensitivity of 0.05ng/ml. The ELISA analytical biochemical technique of the MBS2601701 kit is based on DHVD3 antibody-DHVD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHVD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHVD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DHVD3 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat DHVD3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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  Typical Testing Data/Standard Curve (for reference only) of DHVD3 elisa kit    DHVD3 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2601701. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DHVD3. It may not necessarily be applicable to this product.
 NCBI GI #    306704
 NCBI GeneID    1591
 NCBI Accession #    AAA62379.1 [Other Products]
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 UniProt Related Accession #    Q07973 [Other Products]
 Molecular Weight    43,051 Da
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 NCBI Official Full Name    1,25-dihydroxyvitamin D3 24-hydroxylase
 NCBI Official Synonym Full Names    cytochrome P450, family 24, subfamily A, polypeptide 1
 NCBI Official Symbol    CYP24A1 [Similar Products]
 NCBI Official Synonym Symbols   
CP24; HCAI; CYP24; P450-CC24
[Similar Products]
 NCBI Protein Information    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
 UniProt Protein Name    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
 UniProt Synonym Protein Names   
Cytochrome P450 24A1; Cytochrome P450-CC24
 UniProt Gene Name    CYP24A1 [Similar Products]
 UniProt Synonym Gene Names    CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase [Similar Products]
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 NCBI Summary for DHVD3    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DHVD3    CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.14.13.126; Oxidoreductase

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: steroid metabolic process; osteoblast differentiation; response to vitamin D; vitamin metabolic process; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile
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 Research Articles on DHVD3    1. intermediates of C24-oxidation pathway of 1,25-(OH)2 D3 compete with precursor substrates for binding to the active site of the enzyme, which manifests as an accumulation of intermediates, indicating that they dissociate after each catalytic step.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DHVD3 elisa kitPathways associated with DHVD3 elisa kit
 Reference Product  PubMed Publications
 VDR elisa kit  >169 publications with DHVD3 and VDR
 PTH elisa kit  >19 publications with DHVD3 and PTH
 TLR2 elisa kit  >1 publications with DHVD3 and TLR2
 AHR elisa kit  >1 publications with DHVD3 and AHR
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 Phase 1 - Functionalization Of Compounds Pathway antibodies  Phase 1 - Functionalization Of Compounds Pathway Diagram
Diseases associated with DHVD3 elisa kitOrgans/Tissues associated with DHVD3 elisa kit
 Disease Name  Pubmed Publications
 Hypercalcemia Antibodies  >27 publications with DHVD3 and Hypercalcemia
 Breast Neoplasms Antibodies  >21 publications with DHVD3 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >21 publications with DHVD3 and Prostatic Neoplasms
 Kidney Diseases Antibodies  >21 publications with DHVD3 and Kidney Diseases
 Inflammation Antibodies  >12 publications with DHVD3 and Inflammation
 Cardiovascular Diseases Antibodies  >11 publications with DHVD3 and Cardiovascular Diseases
 Hypercalciuria Antibodies  >10 publications with DHVD3 and Hypercalciuria
 Hypertension Antibodies  >10 publications with DHVD3 and Hypertension
 Renal Insufficiency Antibodies  >10 publications with DHVD3 and Renal Insufficiency
 Neoplasms, Experimental Antibodies  >9 publications with DHVD3 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >82 publications with DHVD3 and Blood
 Kidney Antibodies  >59 publications with DHVD3 and Kidney
 Prostate Antibodies  >32 publications with DHVD3 and Prostate
 Liver Antibodies  >30 publications with DHVD3 and Liver
 Intestine Antibodies  >20 publications with DHVD3 and Intestine
 Skin Antibodies  >18 publications with DHVD3 and Skin
 Brain Antibodies  >6 publications with DHVD3 and Brain
 Embryonic Tissue Antibodies  >6 publications with DHVD3 and Embryonic Tissue
 Mammary Gland Antibodies  >3 publications with DHVD3 and Mammary Gland
 Larynx Antibodies  >1 publications with DHVD3 and Larynx
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