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GCAT elisa kit :: Rat 2-amino-3-ketobutye coenzyme A ligase, mitochondrial (GCAT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7218349 GCAT elisa kit
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

2-amino-3-ketobutye coenzyme A ligase, mitochondrial (GCAT), ELISA Kit

 Also Known As   

Rat 2-amino-3-ketobutye coenzyme A ligase, mitochondrial (GCAT) ELISA Kit

 Product Gene Name   

GCAT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    607422
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 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GCAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GCAT purchase    MBS7218349 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 2-amino-3-ketobutye coenzyme A ligase, mitochondrial (GCAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GCAT. The ELISA analytical biochemical technique of the MBS7218349 kit is based on GCAT antibody-GCAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for GCAT. It may not necessarily be applicable to this product.
 NCBI GI #    7657118
 NCBI GeneID    23464
 NCBI Accession #    NP_055106.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014291.3 [Other Products]
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 UniProt Secondary Accession #    Q6ZWF1; Q96CA9; E2QC23 [Other Products]
 UniProt Related Accession #    O75600 [Other Products]
 Molecular Weight    47,974 Da
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 NCBI Official Full Name    2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform 2
 NCBI Official Synonym Full Names    glycine C-acetyltransferase
 NCBI Official Symbol    GCAT [Similar Products]
 NCBI Official Synonym Symbols   
KBL
[Similar Products]
 NCBI Protein Information    2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase
 UniProt Protein Name    2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
 UniProt Synonym Protein Names   
Aminoacetone synthase; Glycine acetyltransferase
 UniProt Gene Name    GCAT [Similar Products]
 UniProt Synonym Gene Names    KBL; AKB ligase [Similar Products]
 UniProt Entry Name    KBL_HUMAN
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 NCBI Summary for GCAT    The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
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 UniProt Comments for GCAT    GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 2.3.1.29; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: glycine C-acetyltransferase activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; threonine catabolic process to pyruvate; biosynthetic process
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 Research Articles on GCAT    1. Observational study of gene-disease association. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GCAT elisa kitDiseases associated with GCAT elisa kit
 Products by Pathway  Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >1 publications with GCAT and Heart Diseases
 Teratogenesis Antibodies  >1 publications with GCAT and Teratogenesis
 Edema Antibodies  >1 publications with GCAT and Edema
 Congenital Abnormalities Antibodies  >1 publications with GCAT and Congenital Abnormalities
 Inflammation Antibodies  >1 publications with GCAT and Inflammation
 Hemorrhage Antibodies  >1 publications with GCAT and Hemorrhage
 Kidney Diseases Antibodies  >1 publications with GCAT and Kidney Diseases
Organs/Tissues associated with GCAT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with GCAT and Blood
 Kidney Antibodies  >3 publications with GCAT and Kidney
 Bone Antibodies  >3 publications with GCAT and Bone
 Brain Antibodies  >3 publications with GCAT and Brain
 Skin Antibodies  >2 publications with GCAT and Skin
 Eye Antibodies  >2 publications with GCAT and Eye
 Nerve Antibodies  >1 publications with GCAT and Nerve
 Thymus Antibodies  >1 publications with GCAT and Thymus
 Embryonic Tissue Antibodies  >1 publications with GCAT and Embryonic Tissue
 Heart Antibodies  >1 publications with GCAT and Heart
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