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ADAMTS7 elisa kit :: Rat A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024049 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $390 +1 FREE 8GB USB
48-Strip-Wells  /  $580 +1 FREE 8GB USB
96-Strip-Wells  /  $785 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,220 +4 FREE 8GB USB
10x96-Strip-Wells  /  $5,840 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7), ELISA Kit

 Also Known As   

A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit

 Product Synonym Names    COMPase
 Product Gene Name   

ADAMTS7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    605009
 3D Structure    ModBase 3D Structure for Q9UKP4
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7).
No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.058ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of ADAMTS7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAMTS7 purchase    MBS2024049 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing ADAMTS7, with an estimated sensitivity of Typically less than 0.058ng/mL. The ELISA analytical biochemical technique of the MBS2024049 kit is based on ADAMTS7 antibody-ADAMTS7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ADAMTS7 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADAMTS7 elisa kit    ADAMTS7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024049
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NCBI/Uniprot data below describe general gene information for ADAMTS7. It may not necessarily be applicable to this product.
 NCBI GI #    38683827
 NCBI GeneID    11173
 NCBI Accession #    NP_055087.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014272.3 [Other Products]
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 UniProt Primary Accession #    Q9UKP4 [Other Products]
 UniProt Secondary Accession #    Q14F51; Q6P7J9 [Other Products]
 UniProt Related Accession #    Q9UKP4 [Other Products]
 Molecular Weight    184,095 Da
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 NCBI Official Full Name    A disintegrin and metalloproteinase with thrombospondin motifs 7 preproprotein
 NCBI Official Synonym Full Names    ADAM metallopeptidase with thrombospondin type 1 motif, 7
 NCBI Official Symbol    ADAMTS7 [Similar Products]
 NCBI Official Synonym Symbols   
ADAM-TS7; ADAMTS-7; ADAM-TS 7
[Similar Products]
 NCBI Protein Information    A disintegrin and metalloproteinase with thrombospondin motifs 7
 UniProt Protein Name    A disintegrin and metalloproteinase with thrombospondin motifs 7
 UniProt Synonym Protein Names   
COMPase
 Protein Family    A disintegrin and metalloproteinase with thrombospondin motifs
 UniProt Gene Name    ADAMTS7 [Similar Products]
 UniProt Synonym Gene Names    ADAM-TS 7; ADAM-TS7; ADAMTS-7 [Similar Products]
 UniProt Entry Name    ATS7_HUMAN
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 NCBI Summary for ADAMTS7    The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two C-terminal TS motifs. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADAMTS7    ADAMTS7: Metalloprotease that may play a role in the degradation of COMP.

Protein type: Protease; Secreted, signal peptide; Cell surface; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; post-translational protein modification
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 Research Articles on ADAMTS7    1. ADAMTS7 localized to cells having smooth muscle cell markers in human coronary artery disease lesions. Cultured vascular smooth muscle cells had ADAMTS7 at the cytoplasm and cell membrane, where it colocalized with markers of podosomes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADAMTS7 elisa kitPathways associated with ADAMTS7 elisa kit
 Reference Product  PubMed Publications
 PTHLH elisa kit  >1 publications with ADAMTS7 and PTHLH
 FURIN elisa kit  >1 publications with ADAMTS7 and FURIN
 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
Diseases associated with ADAMTS7 elisa kitOrgans/Tissues associated with ADAMTS7 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >11 publications with ADAMTS7 and Cardiovascular Diseases
 Coronary Artery Disease Antibodies  >9 publications with ADAMTS7 and Coronary Artery Disease
 Necrosis Antibodies  >6 publications with ADAMTS7 and Necrosis
 Myocardial Infarction Antibodies  >4 publications with ADAMTS7 and Myocardial Infarction
 Inflammation Antibodies  >4 publications with ADAMTS7 and Inflammation
 Kidney Diseases Antibodies  >2 publications with ADAMTS7 and Kidney Diseases
 Hypertension Antibodies  >2 publications with ADAMTS7 and Hypertension
 Thrombosis Antibodies  >1 publications with ADAMTS7 and Thrombosis
 Urinary Bladder Neoplasms Antibodies  >1 publications with ADAMTS7 and Urinary Bladder Neoplasms
 Hypertrophy Antibodies  >1 publications with ADAMTS7 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >4 publications with ADAMTS7 and Connective Tissue
 Heart Antibodies  >4 publications with ADAMTS7 and Heart
 Kidney Antibodies  >2 publications with ADAMTS7 and Kidney
 Eye Antibodies  >1 publications with ADAMTS7 and Eye
 Skin Antibodies  >1 publications with ADAMTS7 and Skin
 Embryonic Tissue Antibodies  >1 publications with ADAMTS7 and Embryonic Tissue
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