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ABCB1 elisa kit :: Rat ATP-binding cassette, sub-family B (MDR/TAP) , member 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS938959
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
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 Product Name   

ATP-binding cassette, sub-family B (MDR/TAP) , member 1 (ABCB1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Multidrug resistance protein 1, ABCB1 ELISA Kit

 Product Synonym Names    Rat Multidrug resistance protein 1 (ABCB1) ELISA kit; ABC20; CD243; CLCS; GP170; MDR1; MGC163296; P-GP; PGY1; ATP-binding cassette; subfamily B; member 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; multidrug resistance protein 1; ATP-binding cassette; sub-family B (MDR/TAP); member 1
 Product Gene Name   

ABCB1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS938959 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P43245
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat ABCB1. No significant cross-reactivity or interference between rat ABCB1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    <3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ABCB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ABCB1 purchase    MBS938959 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing ABCB1, with an estimated sensitivity of <3.9 pg/ml. The ELISA analytical biochemical technique of the MBS938959 kit is based on ABCB1 antibody-ABCB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABCB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABCB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ABCB1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABCB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ABCB1 elisa kit    ABCB1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS938959. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ABCB1. It may not necessarily be applicable to this product.
 NCBI GI #    1170902
 NCBI GeneID    24646
 NCBI Accession #    P43245.1 [Other Products]
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 UniProt Primary Accession #    P43245 [Other Products]
 UniProt Secondary Accession #    Q63426; Q63427 [Other Products]
 UniProt Related Accession #    P43245 [Other Products]
 Molecular Weight    141,387 Da
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 NCBI Official Full Name    Multidrug resistance protein 1
 NCBI Official Synonym Full Names    ATP-binding cassette, subfamily B (MDR/TAP), member 1B
 NCBI Official Symbol    Abcb1b [Similar Products]
 NCBI Official Synonym Symbols   
Mdr1; Pgy1; Abcb1
[Similar Products]
 NCBI Protein Information    multidrug resistance protein 1; P-glycoprotein 1; P-glycoprotein/multidrug resistance 1; ATP-binding cassette sub-family B member 1; ATP-binding cassette, subfamily B, member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1)
 UniProt Protein Name    Multidrug resistance protein 1
 UniProt Synonym Protein Names   
ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD_antigen: CD243
 Protein Family    ABC transporter B family
 UniProt Gene Name    Abcb1 [Similar Products]
 UniProt Synonym Gene Names    Mdr1; Mdr1b; Pgy1 [Similar Products]
 UniProt Entry Name    MDR1_RAT
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 NCBI Summary for ABCB1    member of the ATP-binding cassette (ABC) protein superfamily; may play a role in drug disposition [RGD, Feb 2006]
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 UniProt Comments for ABCB1    ABCB1B: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Hydrolase; Membrane protein, integral; Transporter, ABC family; EC; Cell adhesion; Transporter

Cellular Component: Golgi membrane; intercellular canaliculus; membrane; mitochondrion; apical plasma membrane; integral to membrane

Molecular Function: protein binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity; drug transporter activity; xenobiotic-transporting ATPase activity; ATP binding

Biological Process: response to drug; lactation; metabolic process; response to arsenic; response to organic cyclic substance; drug transport; transport; response to steroid hormone stimulus; response to hypoxia; multidrug transport; xenobiotic transport; establishment of blood-brain barrier; response to ionizing radiation; transmembrane transport; placenta development
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 Research Articles on ABCB1    1. BM extract decreased the expression of intestinal P-glycoprotein (Pgp) as confirmed by Western blot analysis but did not alter the expression of hepatic Pgp.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ABCB1 elisa kitOrgans/Tissues associated with ABCB1 elisa kit
 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >20 publications with ABCB1 and Liver
 Kidney Antibodies  >10 publications with ABCB1 and Kidney
 Placenta Antibodies  >6 publications with ABCB1 and Placenta
 Heart Antibodies  >3 publications with ABCB1 and Heart
 Small Intestine Antibodies  >1 publications with ABCB1 and Small Intestine
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