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ATP1A1 elisa kit :: Rat ATPase, Na+/K+ transporting, alpha 1 polypeptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS947667
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Sodium/potassium-transporting ATPase subunit alpha-1, ATP1A1 ELISA Kit

 Product Synonym Names    Rat Sodium/potassium-transporting ATPase subunit alpha-1 (ATP1A1) ELISA kit; MGC3285; MGC51750; Na+; K+ ATPase alpha subunit; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na; K-ATPase; alpha-A catalytic polypeptide; Na; K-ATPase alpha-1 subunit; Na; K-ATPase catalytic subunit a; ATPase; Na+/K+ transporting; alpha 1 polypeptide
 Product Gene Name   

ATP1A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS947667 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9N0Z6
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat ATP1A1. No significant cross-reactivity or interference between rat ATP1A1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.2 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of rat ATP1A1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ATP1A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ATP1A1 purchase    MBS947667 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml -2000 pg/ml in biological research samples containing ATP1A1, with an estimated sensitivity of The minimum detectable dose of rat ATP1A1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS947667 kit is based on ATP1A1 antibody-ATP1A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP1A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP1A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ATP1A1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP1A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ATP1A1 elisa kit    ATP1A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS947667. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ATP1A1. It may not necessarily be applicable to this product.
 NCBI GI #    251823702
 NCBI GeneID    100302415
 NCBI Accession #    NP_001156546.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001163074.1 [Other Products]
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 UniProt Primary Accession #    Q9N0Z6 [Other Products]
 UniProt Related Accession #    Q9N0Z6 [Other Products]
 Molecular Weight    112,994 Da
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 NCBI Official Full Name    sodium/potassium-transporting ATPase subunit alpha-1
 NCBI Official Symbol    ATP1A1 [Similar Products]
 NCBI Protein Information    sodium/potassium-transporting ATPase subunit alpha-1; Na/K ATPase alpha 1 subunit; sodium pump subunit alpha-1; Na+/K+ -ATPase alpha 1 subunit; Na(+)/K(+) ATPase alpha-1 subunit
 UniProt Protein Name    Sodium/potassium-transporting ATPase subunit alpha-1
 UniProt Synonym Protein Names   
Sodium pump subunit alpha-1
 Protein Family    Sodium/potassium-transporting ATPase
 UniProt Gene Name    ATP1A1 [Similar Products]
 UniProt Synonym Gene Names    Na(+)/K(+) ATPase alpha-1 subunit [Similar Products]
 UniProt Entry Name    AT1A1_RABIT
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 UniProt Comments for ATP1A1    Function: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients May contribute to blood-heart barrier properties of endocardial endothelium and may control cardiac performance via endothelial Na+/H+ exchange.1 PublicationManual assertion based on experiment in:Ref.1

Catalytic activity: ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).
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 Research Articles on ATP1A1    1. Pb(2+) ions are able to confine the Na(+),K(+)-ATPase into a phosphorylated E(2) state.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ATP1A1 elisa kitOrgans/Tissues associated with ATP1A1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >27 publications with ATP1A1 and Cardiovascular Diseases
 Hypertension Antibodies  >22 publications with ATP1A1 and Hypertension
 Kidney Diseases Antibodies  >10 publications with ATP1A1 and Kidney Diseases
 Neoplasms Antibodies  >7 publications with ATP1A1 and Neoplasms
 Heart Diseases Antibodies  >7 publications with ATP1A1 and Heart Diseases
 Heart Failure Antibodies  >5 publications with ATP1A1 and Heart Failure
 Disease Models, Animal Antibodies  >5 publications with ATP1A1 and Disease Models, Animal
 Carcinoma Antibodies  >4 publications with ATP1A1 and Carcinoma
 Myocardial Ischemia Antibodies  >4 publications with ATP1A1 and Myocardial Ischemia
 Necrosis Antibodies  >3 publications with ATP1A1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >26 publications with ATP1A1 and Heart
 Central Nervous System Antibodies  >5 publications with ATP1A1 and Central Nervous System
 Eye Antibodies  >4 publications with ATP1A1 and Eye
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