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AXIN2 elisa kit :: Rat AXIN2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500216
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AXIN2, ELISA Kit

★Popular Item★
 Also Known As   

Rat AXIN2 (Axis Inhibition Protein 2) ELISA Kit

 Product Gene Name   

AXIN2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    114500
 3D Structure    ModBase 3D Structure for Q9Y2T1
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinantRatAXIN2. No significant cross-reactivity or interference between RatAXIN2 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Min: 0.188ng/mL; Max: 20ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AXIN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AXIN2 purchase    MBS2500216 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AXIN2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing AXIN2, with an estimated sensitivity of Min: 0.188ng/mL; Max: 20ng/mL. The ELISA analytical biochemical technique of the MBS2500216 kit is based on AXIN2 antibody-AXIN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AXIN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AXIN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AXIN2 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatAXIN2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AXIN2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AXIN2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AXIN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AXIN2.You can calculate the concentration of AXIN2in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AXIN2 elisa kit    AXIN2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500216. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AXIN2. It may not necessarily be applicable to this product.
 NCBI GI #    195927059
 NCBI GeneID    8313
 NCBI Accession #    NP_004646.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_004655.3 [Other Products]
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 UniProt Primary Accession #    Q9Y2T1 [Other Products]
 UniProt Secondary Accession #    Q3MJ88; Q9H3M6; Q9UH84 [Other Products]
 UniProt Related Accession #    Q9Y2T1 [Other Products]
 Molecular Weight    93,558 Da
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 NCBI Official Full Name    axin-2
 NCBI Official Synonym Full Names    axin 2
 NCBI Official Symbol    AXIN2 [Similar Products]
 NCBI Official Synonym Symbols   
AXIL; ODCRCS
[Similar Products]
 NCBI Protein Information    axin-2; axin-like protein; axis inhibition protein 2; conductin
 UniProt Protein Name    Axin-2
 UniProt Synonym Protein Names   
Axin-like protein; Axil; Axis inhibition protein 2; Conductin
 Protein Family    Axin
 UniProt Gene Name    AXIN2 [Similar Products]
 UniProt Synonym Gene Names    Axil [Similar Products]
 UniProt Entry Name    AXIN2_HUMAN
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 NCBI Summary for AXIN2    The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AXIN2    axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: Golgi apparatus; centrosome; postsynaptic density; cytoplasmic membrane-bound vesicle; cell cortex; cytosol; beta-catenin destruction complex; microtubule cytoskeleton; nucleoplasm; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; cell proliferation; negative regulation of cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome
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 Research Articles on AXIN2    1. findings indicate that AXIN2 can be regarded as a candidate gene for mutation detection in individuals with non-syndromic oligodontia in the Chinese population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AXIN2 elisa kitPathways associated with AXIN2 elisa kit
 Reference Product  PubMed Publications
 APC elisa kit  >81 publications with AXIN2 and APC
 WNT3A elisa kit  >50 publications with AXIN2 and WNT3A
 AXIN1 elisa kit  >47 publications with AXIN2 and AXIN1
 LRP6 elisa kit  >23 publications with AXIN2 and LRP6
 TCF7L2 elisa kit  >18 publications with AXIN2 and TCF7L2
 GSK3B elisa kit  >2 publications with AXIN2 and GSK3B
 APC2 elisa kit  >1 publications with AXIN2 and APC2
 CSNK1E elisa kit  >1 publications with AXIN2 and CSNK1E
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with AXIN2 elisa kitOrgans/Tissues associated with AXIN2 elisa kit
 Disease Name  Pubmed Publications
 Colorectal Neoplasms Antibodies  >69 publications with AXIN2 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >36 publications with AXIN2 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >17 publications with AXIN2 and Disease Models, Animal
 Liver Diseases Antibodies  >16 publications with AXIN2 and Liver Diseases
 Liver Neoplasms Antibodies  >16 publications with AXIN2 and Liver Neoplasms
 Inflammation Antibodies  >10 publications with AXIN2 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >10 publications with AXIN2 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >10 publications with AXIN2 and Fibrosis
 Lung Neoplasms Antibodies  >8 publications with AXIN2 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >8 publications with AXIN2 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >29 publications with AXIN2 and Embryonic Tissue
 Blood Antibodies  >28 publications with AXIN2 and Blood
 Brain Antibodies  >27 publications with AXIN2 and Brain
 Liver Antibodies  >23 publications with AXIN2 and Liver
 Lung Antibodies  >21 publications with AXIN2 and Lung
 Intestine Antibodies  >19 publications with AXIN2 and Intestine
 Nerve Antibodies  >15 publications with AXIN2 and Nerve
 Connective Tissue Antibodies  >13 publications with AXIN2 and Connective Tissue
 Kidney Antibodies  >12 publications with AXIN2 and Kidney
 Eye Antibodies  >10 publications with AXIN2 and Eye
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