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ATF6 elisa kit :: Rat Activating Transcription Factor 6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533592
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Activating Transcription Factor 6 (ATF6), ELISA Kit

★Popular Item★
 Also Known As   

Rat ATF6 (Activating Transcription Factor 6) ELISA Kit

 Product Gene Name   

ATF6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605537
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinantRatATF6. No significant cross-reactivity or interference between RatATF6 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.625-40ng/mL
 Sensitivity    0.375ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ATF6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ATF6 purchase    MBS2533592 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activating Transcription Factor 6 (ATF6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing ATF6, with an estimated sensitivity of 0.375ng/mL. The ELISA analytical biochemical technique of the MBS2533592 kit is based on ATF6 antibody-ATF6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATF6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATF6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ATF6 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatATF6concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ATF6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ATF6and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ATF6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ATF6.You can calculate the concentration of ATF6in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ATF6 elisa kit    ATF6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2533592. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ATF6. It may not necessarily be applicable to this product.
 NCBI GI #    119611100
 NCBI GeneID    22926
 NCBI Accession #    EAW90694.1 [Other Products]
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 UniProt Secondary Accession #    O15139; Q5VW62; Q6IPB5; Q9UEC9 [Other Products]
 UniProt Related Accession #    P18850 [Other Products]
 Molecular Weight    74,585 Da
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 NCBI Official Full Name    activating transcription factor 6
 NCBI Official Synonym Full Names    activating transcription factor 6
 NCBI Official Symbol    ATF6 [Similar Products]
 NCBI Official Synonym Symbols   
ACHM7; ATF6A
[Similar Products]
 NCBI Protein Information    cyclic AMP-dependent transcription factor ATF-6 alpha
 UniProt Protein Name    Cyclic AMP-dependent transcription factor ATF-6 alpha
 UniProt Synonym Protein Names   
Activating transcription factor 6 alpha; ATF6-alpha
 Protein Family    Cyclic AMP-dependent transcription factor
 UniProt Gene Name    ATF6 [Similar Products]
 UniProt Synonym Gene Names    cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha [Similar Products]
 UniProt Entry Name    ATF6A_HUMAN
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 NCBI Summary for ATF6    This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
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 UniProt Comments for ATF6    ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to endoplasmic reticulum membrane; membrane; nuclear envelope; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; ubiquitin protein ligase binding

Biological Process: cellular protein metabolic process; eye development; positive regulation of transcription of target genes involved in unfolded protein response; protein folding; regulation of transcription from RNA polymerase II promoter; response to stress; signal transduction; transcription, DNA-dependent; unfolded protein response; visual perception

Disease: Achromatopsia 7
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 Research Articles on ATF6    1. These results confirm that HIV infection activates stress-response components and that antiretroviral therapy contributes to changes in the unfolded protein response activation profile.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ATF6 elisa kitPathways associated with ATF6 elisa kit
 Reference Product  PubMed Publications
 DDIT3 elisa kit  >239 publications with ATF6 and DDIT3
 XBP1 elisa kit  >199 publications with ATF6 and XBP1
 ATF4 elisa kit  >180 publications with ATF6 and ATF4
 ERN1 elisa kit  >70 publications with ATF6 and ERN1
 HSPA5 elisa kit  >37 publications with ATF6 and HSPA5
 YY1 elisa kit  >7 publications with ATF6 and YY1
 MBTPS2 elisa kit  >4 publications with ATF6 and MBTPS2
 WFS1 elisa kit  >3 publications with ATF6 and WFS1
 MAPK14 elisa kit  >2 publications with ATF6 and MAPK14
 HSP90B1 elisa kit  >1 publications with ATF6 and HSP90B1
 Products by Pathway  Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 ATF6-alpha Activates Chaperone Genes Pathway antibodies  ATF6-alpha Activates Chaperone Genes Pathway Diagram
 ATF6-alpha Activates Chaperones Pathway antibodies  ATF6-alpha Activates Chaperones Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
Diseases associated with ATF6 elisa kitOrgans/Tissues associated with ATF6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >69 publications with ATF6 and Inflammation
 Liver Diseases Antibodies  >61 publications with ATF6 and Liver Diseases
 Brain Diseases Antibodies  >49 publications with ATF6 and Brain Diseases
 Necrosis Antibodies  >40 publications with ATF6 and Necrosis
 Heart Diseases Antibodies  >29 publications with ATF6 and Heart Diseases
 Lung Diseases Antibodies  >26 publications with ATF6 and Lung Diseases
 Fatty Liver Antibodies  >22 publications with ATF6 and Fatty Liver
 Fibrosis Antibodies  >22 publications with ATF6 and Fibrosis
 Atherosclerosis Antibodies  >16 publications with ATF6 and Atherosclerosis
 Hypertrophy Antibodies  >12 publications with ATF6 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >109 publications with ATF6 and Liver
 Brain Antibodies  >84 publications with ATF6 and Brain
 Blood Antibodies  >81 publications with ATF6 and Blood
 Muscle Antibodies  >68 publications with ATF6 and Muscle
 Heart Antibodies  >56 publications with ATF6 and Heart
 Vascular Antibodies  >54 publications with ATF6 and Vascular
 Pancreas Antibodies  >41 publications with ATF6 and Pancreas
 Lung Antibodies  >34 publications with ATF6 and Lung
 Bone Antibodies  >30 publications with ATF6 and Bone
 Eye Antibodies  >30 publications with ATF6 and Eye
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